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Handbook of Microbiological Media, Fourth Edition part 122

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Handbook of Microbiological Media, Fourth Edition part 122 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Modified FWM Medium 1205 Solution G.10.0mL Solution B.1.0mL Solution C.1.0mL Solution D.1.0mL pH 7.3 0.2 at 25 C Solution A Composition per 940.0mL NaCl.1.0g KCl.0.5g MgCl2-6H2O.0.4g NH4Cl.0.25g KH2PO4.0.2g CaCl22H2O.0.15g Resazurin.0.5mg Preparation of Solution A Prepare under 80 N2 20 CO2 gas atmosphere. Add components to distilled deionized water and bring volume to 940.0mL. Mix thoroughly. Adjust pH to 7.2. Sparge with 80 N2 20 CO2. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 25 C. Solution B Composition per liter FeCl24H2O.1.5g CoCl26H2O.190.0mg MnCl24H20.100.0mg ZnCl2.70.0mg Na2MoO4-2H2O.36.0mg NiCl26H2O.24.0mg H3BO3.6.0mg CuCl22H2O.2.0mg HCl 25 solution .10.0mL Preparation of Solution B Add FeCl2-4H2O to 10.0mL of HCl solution. Mix thoroughly. Add distilled deionized water and bring volume to 1.0L. Add remaining components. Mix thoroughly. Sparge with 80 N2 20 CO2. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 25 C. Solution C Composition per liter Pyridoxine hydrochloride.300.0mg Thiamine-HCl-2H2O.200.0mg Nicotinic acid.200.0mg Vitamin B12.100.0mg Calcium pantothenate.100.0mg p-Aminobenzoic acid.80.0mg D -Biotin.20.0mg Preparation of Solution C Add components to distilled deionized water and bring volume to 1.0L. Sparge with 100 N2. Mix thoroughly. Filter sterilize. Solution D Composition per liter NaOH.0.5g Na2WO4-2H2O.4.0mg Na2SeO3-5H2O.3.0mg Preparation of Solution D Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 100 N2. Filter sterilize. Solution E Composition per 100.0mL NaHCO3.5.0g Preparation of Solution E Add NaHCO3 to distilled deionized water and bring volume to 100.0mL. Mix thoroughly. Sparge with 100 N2 gas mixture. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 25 C. Solution F Composition per 10.0mL Fructose. 2.0g Preparation of Solution F Add fructose to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100 N2 gas mixture. Autoclave