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Handbook of Microbiological Media, Fourth Edition part 128

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Handbook of Microbiological Media, Fourth Edition part 128 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Mycoplasma Broth 1265 Basal Medium Composition per 700.0mL Sorbitol.50.0g Beef heart solids from infusion.16.2g Peptone.3.26g NaCl.1.62g Fructose.1.0g Glucose.1.0g Sucrose.1.0g Pancreatic digest of casein.1.0g Preparation of Basal Medium Add components to distilled de-ionized water and bring volume to 700.0mL. Mix thoroughly. Adjust pH to 7.5-7.8. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 50 C. Fresh Yeast Extract Solution Composition per 100.0mL Baker s yeast live pressed starch-free.25.0g Preparation of Fresh Yeast Extract Solution Add the live Baker s yeast to 100.0mL of distilled deionized water. Autoclave for 90 min at 15 psi pressure-121 C. Allow to stand. Remove supernatant solution. Adjust pH to 6.6-6.8. Preparation of Medium Filter sterilize horse serum and fresh yeast extract solution. Aseptically add to cooled sterile basal medium. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use For the cultivation and maintenance of Mycoplasma mycoides Spiroplasma apis Spiroplasma citri and Spiroplasma melliferum. Mycoplasma Broth Composition per 950.0mL Glucose.1.0g Nicotinamide adenine dinucleotide.0.1g PPLO broth without Crystal Violet.680.0mL Swine serum 56 C 30 min .150.0mL Fresh yeast extract solution.100.0mL Phenol Red 0.1 w v solution .20.0mL pH 7.8 0.2 at 25 C PPLO Broth without Crystal Violet Composition per 680.0mL Beef heart solids from infusion.11.3g Peptone.2.28g NaCl.1.13g Source PPLO broth without Crystal Violet is available as a premixed powder from BD Diagnostic Systems. Preparation of PPLO Broth without Crystal Violet Add components to distilled deionized water and bring volume to 680.0mL. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 56 C. Fresh Yeast Extract Solution Composition per 100.0mL Baker s yeast live pressed starch-free.25.0g Preparation of Fresh Yeast Extract Solution Add the live Baker s yeast to 100.0mL of distilled deionized water. Autoclave for 90 min at 15 psi pressure-121 C. Allow to .