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Handbook of Microbiological Media, Fourth Edition part 130

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Handbook of Microbiological Media, Fourth Edition part 130 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Neurospora Minimal Medium 1285 Vitamin Solution Composition per liter Ribonucleic acid alkali hydrolyzed.0.5g Inositol.0.4g Choline.0.2g Nicotinamide.0.2g Pantothenic acid.0.2g Thiamine.0.1g p-Aminobenzoic acid.0.05g Pyridoxine.0.05g Riboflavin.0.05g Folic acid.4.0pg Preparation of Vitamin Solution Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except vitamin solution to distilled deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Aseptically add 10.0mL of sterile vitamin solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use For the cultivation of Neurospora species on complete medium. Neurospora Medium Composition per liter Sucrose.15.0g Ammonium tartrate.5.0g KH2PO4.1.0g NH4NO3.1.0g MgSO4-7H2O.0.5g CaCl2.0.1g NaCl.0.1g ZnCl2.2.0mg FeCl3.0.2mg Cu Cl2.0.1mg MnCl2.0.02mg Na2MoO4-2H2O.0.02mg H3BO3.0.01mg Biotin.1.0pg Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the cultivation of Neurospora species on minimal medium. Neurospora Minimal Medium Composition per liter Sucrose.20.0g Ammonium tartrate.5.0g KH2PO4.1.0g NaNO3.1.0g MgSO4-7H2O.0.5g CaCl22H2O.0.1g NaCl.0.1g ZnSO4-7H2O.5.5mg FeSO4-7H2O.0.54mg CuSO4-5H2O.0.39mg MnSO4-4H2O.0.063mg H3BO3 . 0.057mg Na2MoO4-2H2O.0.05mg Biotin.5.0pg pH 7.3 0.2 at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the cultivation of Neurospora species and the detection of Neurospora mutants. Neurospora Minimal Medium Composition per liter Sucrose.20.0g Agar.20.0g .