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Handbook of Microbiological Media, Fourth Edition part 136

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Handbook of Microbiological Media, Fourth Edition part 136 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Park and Sanders Enrichment Hi Veg Broth Base with Horse Blood and Selective Antibiotics 1345 Nicotinamide.0.5g Pyridoxal-HCl.0.5g Riboflavin.0.5g Folic acid.0.5g a-Lipoic acid.0.1g Biotin.0.1mg Preparation of Supplement A Add components to distilled deionized water and bring volume to 5.0mL. Mix thoroughly. Filter sterilize. Supplement B Composition per 5.0mL Preparation of Solution A Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Distribute while stirring into screw-capped tubes in 10.0mL volumes. Store at -20 C. Cefoperazone.0.032g Cycloheximide. 0.1g Thaw as needed. Solution B Composition per 100.0mL TEM-4T Hachmeister Pittsburgh .10.0g Stigmasterol.0.5g Ethanol absolute.100.0mL Preparation of Solution B Add TEM-4T and stigmasterol to 100.0mL of hot ethanol. Mix thoroughly. Store at 4 C. Solution C Composition per 500.0mL Proteose peptone.10.0g Pancreatic digest of casein.5.0g Ribonucleic acid.1.0g MgSO4-7H2O.0.5g Preparation of Solution C Add components to distilled deionized water and bring volume to 500.0mL. Mix thoroughly. Preparation of Medium Combine 500.0mL of solution C 10.0mL of solution A and 1.0mL of solution B. Mix thoroughly. Bring volume to 1.0L with distilled deionized water. Adjust pH to 7.0-7.2 with 0.1 N NaOH. Autoclave for 20 min at 15 psi pressure-121 C. Cool to 45 -50 C. Aseptically distribute into sterile tubes or flasks. Use For the cultivation ofParamecium species to be used as host cells by bacterial symbionts. Park and Sanders Enrichment Broth Composition per 1010.0mL Preparation of Supplement B Add components to distilled deionized water and bring volume to 5.0mL. Mix thoroughly. Filter sterilize. Caution Cycloheximide is toxic. Avoid skin contact or aerosol formation and inhalation. Preparation of Medium To 1.0L of cooled sterile basal medium aseptically add 5.0mL of sterile supplement A. Mix thoroughly. Aseptically distribute into flasks in 100.0mL volumes. Inoculate medium with food samples. .