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Handbook of Microbiological Media, Fourth Edition part 154

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Handbook of Microbiological Media, Fourth Edition part 154 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | S Medium 1525 Ryan s Aeromonas Medium See Aeromonas Medium R2YE Medium Composition per 1062.2mL Thiostrepton.50.0mg Basal solution.800.0mL TES jV-tris hydroxymethyl methyl-2-amino-ethane- sulfonic acid buffer.100.0mL CaCl2-2H2O solution.80.2mL Yeast extract solution.50.0mL L-Proline solution.15.0mL KH2PO4 solution.10.0mL NaOH solution.5.0mL Trace elements solution.2.0mL pH 7.2 0.2 at 25 C Basal Solution Composition per 800.0mL Sucrose.103.0g MgCl26H20.10.12g D-Glucose.10.0g K2SO4.0.25g Casamino acids.0.1g Preparation of Basal Solution Add components to distilled de-ionized water and bring volume to 800.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 25 C. TES Buffer Composition per liter TES jV-tris hydroxymethyl methyl-2-amino-ethane- sulfonic acid buffer.57.3g Preparation of TES Buffer Add TES to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2. Filter sterilize. CaCl2-2H2O Solution Composition per 100.0mL CaCl22H2O.3.68g Preparation of CaCl2-2H2O Solution Add the CaCl22H2O to distilled deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Yeast Extract Solution Composition per 100.0mL Yeast extract.10.0g Preparation of Yeast Extract Solution Add yeast extract to dis-tilled deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. L-Proline Solution Composition per 100.0mL L-Proline.20.0g Preparation of L-Proline Solution Add the proline to distilled deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. KH2PO4 Solution Composition per 100.0mL KH2PO4.0.5g Preparation of KH2PO4 Solution Solution Add the KH2PO4 to distilled deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. NaOH Solution Composition per 100.0mL NaOH. 40.0g Preparation of NaOH Solution Add the NaOH to distilled deion-ized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize Trace Elements Solution Composition per liter .