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Handbook of Microbiological Media, Fourth Edition part 164

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Handbook of Microbiological Media, Fourth Edition part 164 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | SSL Agar 1625 NaHCO3 Solution Composition per 100.0mL NaHCO3.10.0g Preparation of NaHCO3 Solution Add NaHCO3 to distilled de-ionized water and bring volume to 100.0mL. Mix thoroughly. Sparge with 80 N2 20 CO2. Filter sterilize. NH4Cl Solution Composition per 100.0mL NH4Cl.0.5g Preparation of NH4CI Solution Add NH4Cl to distilled deion-ized water and bring volume to 100.0mL. Mix thoroughly. Sparge with 100 N2. Filter sterilize. KH2PO4 Solution Composition per 100.0mL KH2PO4.0.4g Preparation of KH2PO4 Solution Add KH2PO4 to distilled de-ionized water and bring volume to 100.0mL. Mix thoroughly. Sparge with 100 N2. Filter sterilize. Substrate Solution Composition per 10.0mL Na-propionate.1.5g Preparation of Substrate Solution Add Na-propionate to dis-tilled deionized water and bring volume to 10.0mL. Sparge with N2. Filter sterilize. Preparation of Medium Prepare and dispense medium under 80 N2 20 CO2. Add components except NaHCO3 solution Na2S-9H2O solution substrate solution selenite-tungstate solution NH4Cl solution KH2PO4 solution vitamin solution dithionite solution and trace elements solution SL-10. Distribute 30.0mL aliquots into 50mL serum bottles. Autoclave for 15 min at 15 psi pressure-121 C. Cool to room temperature. Aseptically and anaerobically for each 30.0mL medium add 0.9mL NaHCO3 solution 0.3mL Na2S-9H2O solution 0.3mL substrate solution 0.03mL selenite-tungstate solution 1.5mL NH4Cl solution 1.5mL KH2PO4 solution 0.3mL vitamin solution 0.03mL dithionite solution and 0.03mL trace elements solution SL-10. Final pH is 7.2. Use For the cultivation of Desulfofaba gelida DSM 12344. SS Agar See Salmonella Shigella Agar SS Agar HiVeg Salmonella Shigella Agar HiVeg Composition per liter Agar.15.0g Plant peptone.11.5g Lactose.10.0g Sodium citrate.10.0g Na2S2O3.8.5g Plant extract.5.0g Synthetic detergent No. I.2.0g Ferric citrate.1.0g Neutral Red.0.025g Brilliant Green.0.33mg pH 7.0 0.2 at 25 C Source This medium is available as a premixed powder from Hi-Media.