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Handbook of Microbiological Media, Fourth Edition part 171

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Handbook of Microbiological Media, Fourth Edition part 171 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Termitobacter Medium 1695 to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Pour into sterile Petri dishes or leave in tubes. Use For the selective isolation and differentiation of enteric bacteria from urine. Tergitol 7 Broth Composition per liter Lactose.10.0g Yeast extract.3.0g Pancreatic digest of casein.2.5g Peptic digest of animal tissue.2.5g Tergitol 7.0.1g Bromthymol Blue.25.0mg TTC solution.3.0mL pH 6.9 0.2 at 25 C Source This medium is available as a premixed powder from BD Diagnostic Systems. TTC Solution Composition per 100.0mL Triphenyltetrazolium chloride.1.0g Preparation of TTC Solution Add triphenyltetrazolium chloride to distilled deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium Add components to distilled deionized water and bring volume to 997.0mL. Mix thoroughly. Gently heat while stirring and bring to boiling. Autoclave for 15 min at 15 psi pres-sure-121 C. Cool to 25 C. Aseptically add 3.0mL of sterile TTC solution. Mix thoroughly. Use For the isolation and cultivation of coliforms Salmonella and other enteric bacteria. Tergitol 7 HiVeg Agar Base with TTC Composition per liter Agar.15.0g Lactose.10.0g Plant peptone No. 3.5.0g Yeast extract.3.0g Sodium heptadecyl sulfate.0.1g Bromthymol Blue.0.025g TTC solution.5.0mL pH 7.2 0.2 at 25 C Source This medium without TTC solution is available as a premixed powder from HiMedia. TTC Solution Composition per 100.0mL Triphenyltetrazolium chloride.0.05g Preparation of TTC Solution Add triphenyltetrazolium chloride to distilled deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium Add components to distilled deionized water and bring volume to 995.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 50 C. Aseptically add 5.0mL of sterile TTC solution. Mix thoroughly. Pour into sterile Petri dishes or distribute