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Handbook of Microbiological Media, Fourth Edition part 172

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Handbook of Microbiological Media, Fourth Edition part 172 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Thauera aromatica AR-1 Medium 1705 Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the cultivation and maintenance of Demococcus species. Thauera aromatica AR-1 Medium DSMZ Medium 855 Composition per 992.0mL Solution A.870.0mL Solution C.100.0mL Solution D.10.0mL Solution E Vitamin solution .10.0mL Solution B Trace elements solution SL-10 .1.0mL Selenite-tungstate solution.1.0mL pH 7.2 0.2 at 25 C Solution A Composition per 870.0mL Na2SO4.3.0g NaCl.1.0g KNO3.0.6g KCl.0.5g MgCl2-6H2O.0.4g NH4Cl.0.3g KH2PO4.0.2g CaCl22H2O.0.15g Resazurin.1.0mg Preparation of Solution A Add components to distilled deionized water and bring volume to 870.0mL. Mix thoroughly. Solution B Trace Elements Solution SL-10 Composition per liter FeCl24H2O.1.5g CoCl26H2O.190.0mg MnCl24H2O.100.0mg ZnCl2.70.0mg Na2MoO4-2H2O.36.0mg NiCl2-6H2O.24.0mg H3BO3.6.0mg CuCl22H2O.2.0mg HCl 25 solution .10.0mL Preparation of Solution B Trace Elements Solution SL-10 Add FeCl2-4H2O to 10.0mL of HCl solution. Mix thoroughly. Add distilled deionized water and bring volume to 1.0L. Add remaining components. Mix thoroughly. Sparge with 100 N2. Autoclave for 15 min at 15 psi pressure-121 C. Solution C Composition per 100.0mL NaHCO3.5.0g Preparation of Solution C Add NaHCO3 to distilled deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Flush with 80 N2 20 CO2 to remove dissolved oxygen. Solution D Composition per 10.0mL Na-benzoate.0.7g Preparation of Solution D Add Na-benzoate to distilled deion-ized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100 N2. Autoclave for 15 min at 15 psi pressure-121 C. Solution E Vitamin Solution Composition per liter Pyridoxine-HCl.10.0mg Thiamine-HCl-2H2O.5.0mg Riboflavin.5.0mg Nicotinic acid.5.0mg D-Ca-pantothenate.5.0mg p-Aminobenzoic acid.5.0mg Lipoic acid.5.0mg Biotin.2.0mg Folic acid . .