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Handbook of Microbiological Media, Fourth Edition part 173

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Handbook of Microbiological Media, Fourth Edition part 173 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Thermoanaerobacter tengcongensis Medium 1715 KCl.0.33g KH2PO4.0.33g MgCl2-6H2O.0.33g CaCl22H2O.0.33g Resazurin.0.5mg Glucose solution.25.0mL Na2S-9H2O solution.15.0mL Vitamin solution.10.0mL Yeast extract solution.5.0mL Trace elements solution SL-10.1.0mL pH 7.0 0.2 at 25 C Vitamin Solution Composition per liter Pyridoxine-HCl.10.0mg Thiamine-HCl-2H2O.5.0mg Riboflavin.5.0mg Nicotinic acid.5.0mg D-Ca-pantothenate.5.0mg p-Aminobenzoic acid.5.0mg Lipoic acid.5.0mg Biotin.2.0mg Folic acid.2.0mg Vitamin B12 . 0.1mg Preparation of Vitamin Solution Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80 H2 20 CO2. Filter sterilize. Glucose Solution Composition per 50.0mL Glucose.10.0g Preparation of Glucose Solution Add glucose to distilled deion-ized water and bring volume to 50.0mL. Mix thoroughly. Filter sterilize. Yeast Extract Solution Composition per 10.0mL Yeast extract.1.0g Preparation of Yeast Extract Solution Add yeast extract to dis-tilled deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave under 100 N2 for 15 min at 15 psi pressure-121 C. Cool to room temperature. Trace Elements Solution SL-10 Composition per liter FeCl24H2O.1.5g CoCl26H2O.190.0mg MnCl24H2O.100.0mg ZnCl2.70.0mg Na2MoO4-2H2O.36.0mg NiCl2-6H2O.24.0mg H3BO3.6.0mg CuCl22H2O.2.0mg HCl 25 solution .10.0mL Preparation of Trace Elements Solution SL-10 Add FeCl24H2O to 10.0mL of HCl solution. Mix thoroughly. Add distilled deionized water and bring volume to 1.0L. Add remaining components. Mix thoroughly. Sparge with 80 N2 20 CO2. Autoclave for 15 min at 15 psi pressure-121 C. Na2S-9H2O Solution Composition per 20.0mL Na2S-9H2O.0.6g Preparation of Na2S-9H2O Solution Add Na2S-9H2O to dis-tilled deionized water and bring volume to 20.0mL. Mix thoroughly. Autoclave under 100 N2 for 15 min at 15 psi pressure-121 C. Cool to room temperature. Preparation of Medium Prepare and dispense medium under 80 N2 20 CO2 gas mixture. Add components except .