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Handbook of Microbiological Media, Fourth Edition part 174

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Handbook of Microbiological Media, Fourth Edition part 174 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Thermodesulfotobacterium Agar 1725 Na2S-9H2O solution.10.0mL Na2S2O4 solution.10.0mL Trace elements solution SL-10.1.0mL NaHCO3 solution.variable pH 6.8 0.2 at 25 C NaHCO3 Solution Composition per 20.0mL NaHCO3.1.0g Preparation of NaHCO3 Solution Add NaHCO3 to distilled de-ionized water and bring volume to 20.0mL. Mix thoroughly. Sparge with 80 N2 20 CO2. Autoclave for 15 min at 15 psi pressure-121 C. Na2S-9H2O Solution Composition per 10.0mL Na2S-9H2O.0.15g Preparation of Na2S-9H2O Solution Add Na2S-9H2O to dis-tilled deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100 N2. Autoclave for 15 min at 15 psi pressure-121 C. Na2S2O3 Solution Composition per 10.0mL Na2S2O3-5H2O.2.0g Preparation of Na2S2O3 Solution Add Na2S2O3-5H2O to dis-tilled deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100 N2. Autoclave for 15 min at 15 psi pressure-121 C. Trace Elements Solution SL-10 Composition per liter FeCl24H2O.1.5g CoCl26H2O.190.0mg MnCl24H20.100.0mg ZnCl2.70.0mg Na2MoO4-2H2O.36.0mg NiCl26H2O.24.0mg H3BO3.6.0mg CuCl22H2O.2.0mg HCl 25 solution .10.0mL Preparation of Trace Elements Solution SL-10 Add FeCl2-4H2O to 10.0mL of HCl solution. Mix thoroughly. Add distilled deionized water and bring volume to 1.0L. Add remaining components. Mix thoroughly. Preparation of Medium Prepare and dispense medium under 100 N2. Add components except Na2S-9H2O solution Na2S2O3 solution and NaHCO3 solution to distilled deionized water and bring volume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling. Cool to room temperature while sparging with 100 N2. Anaerobically distribute 9.8mL volumes into tubes. Autoclave for 15 min at 15 psi pres-sure-121 C. Aseptically and anaerobically add 0.1mL of sterile Na2S-9H2O solution and 0.1mL of sterile Na2S2O4 solution to each tube. Aseptically and anaerobically add a sufficient volume of sterile NaHCO3 solution to each tube to bring the pH to 6.8. Use For the cultivation of Thermodesulforhabdus .