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Handbook of Microbiological Media, Fourth Edition part 175

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Handbook of Microbiological Media, Fourth Edition part 175 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Thermosipho africanus Medium 1735 Glucose Solution Composition per 10.0mL Glucose.5.0g Preparation of Glucose Solution Add glucose to distilled deion-ized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Yeast Extract Solution Composition per 10.0mL Yeast extract.1.0g Preparation of Yeast Extract Solution Add yeast extract to dis-tilled deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except glucose solution and yeast extract solution to distilled deionized water and bring volume to 980.0mL. Mix thoroughly. Adjust pH to 2.0 with 10A H2SO4. Autoclave for 15 min at 15 psi pressure-121 C. Aseptically add 10.0mL of sterile glucose solution and 10.0mL of sterile yeast extract solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Final pH should be 2.0-3.0. Use For the aerobic cultivation and maintenance of Thermoplasma volcanium. Thermoproteus Medium Composition per liter Solution A.500.0mL Solution B.450.0mL Solution C.50.0mL pH 4.8-5.6 at 25 C Solution A Composition per 500.0mL Glucose.10.0g FeSO4-7H2O.0.556g MgSO4-7H2O.0.492g CaSO4-2H2O.0.344g NH4 2SO4.0.264g Yeast extract.0.2g KH2PO4.0.014g Resazurin.1.0mg Trace elements.10.0mL Preparation of Solution A Add components to distilled deionized water and bring volume to 500.0mL. Mix thoroughly. Immediately filter sterilize. Trace Elements Composition per liter Na2B4O7-10H2O.0.45g MnCl24H2O.0.18g ZnSO4-7H2O.0.022g CuCl22H2O.5.0mg Na2MoO4-2H2O.3.6mg VOSO4-5H2O.3.6mg CoSO4-7H2O.1.2mg Preparation of Trace Elements Add components to distilled de-ionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 3.0 with H2SO4 to retard precipitation. Solution B Composition per 450.0mL Sulfur.10.0g Preparation of Solution B Add sulfur to 450.0mL of distilled deionized water. Autoclave for 30 min at 0 psi pressure-100 C on 3 consecutive days. Solution C Composition per 50.0mL Na2S-9H2O.0.85g Preparation of Solution C