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Handbook of Microbiological Media, Fourth Edition part 176

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Handbook of Microbiological Media, Fourth Edition part 176 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Thermus Enhanced Agar with 1 Sodium Chloride 1745 Trace Elements Solution Composition per liter MnSO4-H2O.2.2g H3BO3.0.5g ZnSO4-7H2O.0.5g CoCl26H2O.46.0mg Na2MoO4-2H2O.25.0mg CuSO4.16.0mg H2SO4.0.5mL Preparation of Trace Elements Solution Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the cultivation of Thermus brockii. Thermus Broth Composition per liter Pancreatic digest of casein.2.5g Yeast extract.2.5 g Na2HPO4-12H2O.0.43g MgCl26H2O.0.2g Nitrilotriacetic acid.0.1g KH2PO4.54.0mg CaSO4-2H2O.40.0mg Micronutrient solution.1.0mL Fe-citrate solution.0.5mL pH 7.2 0.2 at 25 C Micronutrient Solution Composition per liter MnSO4-H2O.2.28g H3BO3.0.5g ZnSO4-7H2O.0.5g CoCl26H2O.45.0mg CuSO4-5H2O.25.0mg Na2MoO4 2H2O.25.0mg H2SO4 concentrated.0.5mL Preparation of Micronutrient Solution Add components to dis- tilled deionized water and bring volume to 1.0L. Mix thoroughly. Fe-Citrate Solution Composition per liter Fe-citrate.24.5mg Preparation of Fe-Citrate Solution Add Fe-citrate to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Preparation of Medium Add nitrilotriacetic acid to 100.0mL of distilled deionized water. Mix thoroughly. Adjust pH to 6.5 with KOH. Add remaining components and bring volume to 1.0L with distilled deionized water. Mix thoroughly. Adjust pH to 7.2. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Use For the cultivation and maintenance of Thermus species. Thermus Enhanced Agar Composition per liter Agar.28.0g Pancreatic digest of casein.2.5g Yeast extract.2.5 g MgCl26H2O.0.2g Nitrilotriacetic acid.0.1g CaSO4-2H2O.40.0mg Phosphate buffer solution.100.0mL Ferric citrate solution.0.5mL Trace elements solution.0.5mL Phosphate Buffer Solution Composition per liter .