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Handbook of Microbiological Media, Fourth Edition part 187

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Handbook of Microbiological Media, Fourth Edition part 187 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Tween 80A Medium 1855 Ascorbic acid.0.2g Bovine serum heat inactivated.50.0mL Hemin solution.20.0mL Hemin Solution Composition per 50.0mL Hemin.50.0mg Preparation of Hemin Solution Add 50.0mg of hemin to dis-tilled deionized water and bring volume 50.0mL. Adjust the pH to 10.5 with 1jV NaOH. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except tryptose hemin solution and bovine serum to distilled deionized water and bring volume to 600.0mL. Mix thoroughly. Add tryptose. Mix thoroughly. Gently heat and bring to boiling. Cool to 25 C. Autoclave for 15 min at 15 psi pressure-121 C. Aseptically add 50.0mL of sterile heat-inactivated bovine serum and 20.0mL of sterile hemin solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use For the cultivation of Crithidia fasciculata. TweenTM 80A Agar Composition per liter Agar.15.0g Pancreatic digest of casein.5.0g Yeast extract.3.0g MgSO4-7H2O.2.0g Tween 80 solution.50.0mL pH 7.2 0.2 at 25 C Tween 80 Solution Composition per 50.0mL Tween 80.10.0g Preparation of Tween 80 Solution Add Tween 80 to distilled deionized water and bring volume to 50.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Preparation of Medium Add components except Tween 80 solution to distilled deionized water and bring volume to 950.0mL. Gently heat and bring to boiling. Adjust pH to 7.2. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 50 -55 C. Aseptically add 50.0mL of sterile Tween 80 solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use For the cultivation of Agitococcus lubricus. Tween 80 Agar See Polysorbate 80 Agar Tween 80 Agar DSMZ Medium 884 Composition per liter Solution A.900.0mL Solution B.100.0mL Solution A Composition per 900.0mL Agar.15.0g Peptone.10.0g NaCl.5.0g CaCl2-2H2O.0.1g pH 7.1 0.2 at 25 C Preparation of Solution A Add components to distilled deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and