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Handbook of Microbiological Media, Fourth Edition part 192

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Handbook of Microbiological Media, Fourth Edition part 192 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Wilkins-Chalgren Anaerobe Agar with GN Supplement 1905 K2HPO4.0.5g MgSO4-7H2O.0.25g Na2SO3 anhydrous.0.05g pH 7.2 0.2 at 25 C Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Pour into sterile Petri dishes or leave in tubes. Use For the cultivation and maintenance ofXanthomonas albilineans and other Xanthomonas species. Wilkins-Chalgren Agar Composition per liter Agar.15.0g Gelatin peptone.10.0g Pancreatic digest of casein.10.0g NaCl.5.0g Yeast extract.5.0g Glucose.1.0g L-Arginine.1.0g Sodium pyruvate.1.0g Hemin.5.0mg Vitamin K1 menadione .0.5mg pH 7.1 0.2 at 25 C Source This medium is available as a premixed powder from BD Diagnostic Systems. Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 50 -55 C. Add antibiotic to be assayed varying concentrations of antibiotics are used. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes. Use For the cultivation and maintenance of anaerobic bacteria. For standardized antimicrobic susceptibility testing to determine the minimum inhibitory concentrations of antimicrobics for anaerobic bacteria. Wilkins-Chalgren Anaerobe Agar Composition per liter Agar.10.0g Pancreatic digest of casein.10.0g Gelatin peptone.10.0g NaCl.5.0g Yeast extract.5.0g Glucose.1.0g L-Arginine.1.0g Sodium pyruvate.1.0g Hemin.5.0mg Menadione.0.5mg Defibrinated blood.50.0mL Tween 80.1.0mL pH 7.1 0.2 at 25 C Source This medium is available as a premixed powder from Oxoid Unipath. Preparation of Medium Add components except defibrinated blood to distilled deionized water and bring volume to 950.0mL. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 .