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Báo cáo sinh học: "A global analysis of genetic interactions in Caenorhabditis elegans"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: A global analysis of genetic interactions in Caenorhabditis elegans. | Journal of Biology BioMed Central Research article Open Access A global analysis of genetic interactions in Caenorhabditis elegans Alexandra B Byrne 1 Matthew T Weirauch Victoria Wong Martina Koeva Scott J Dixon 1 Joshua M Stuart and Peter J Roy Addresses Department of Medical Genetics and Microbiology The Terrence Donnelly Centre for Cellular and Biomolecular Research 160 College St University of Toronto Toronto ON M5S 3E1 Canada. Collaborative Program in Developmental Biology University of Toronto Toronto ON M5S 3E1 Canada. Department of Biomolecular Engineering 1156 High Street Mail Stop SOE2 University of California Santa Cruz CA 95064 USA. Correspondence Peter J Roy. Email peter.roy@utoronto.ca Joshua M Stuart. Email jstuart@soe.ucsc.edu Received 4 June 2007 Revised 31 July 2007 Accepted 17 August 2007 Published 26 September 2007 Journal of Biology 2007 6 8 The electronic version of this article is the complete one and can be found online at http jbiol.com content 6 3 8 2007 Byrne et al. licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Understanding gene function and genetic relationships is fundamental to our efforts to better understand biological systems. Previous studies systematically describing genetic interactions on a global scale have either focused on core biological processes in protozoans or surveyed catastrophic interactions in metazoans. Here we describe a reliable high-throughput approach capable of revealing both weak and strong genetic interactions in the nematode Caenorhabditis elegans. Results We investigated interactions between II query mutants in conserved signal transduction pathways and hundreds of target genes compromised by RNA interference RNAi . Mutant-RNAi combinations .