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Báo cáo lâm nghiệp: "Polyamines during somatic embryo development in Norway spruce (Picea abies [L.])"

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Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí lâm nghiệp đề tài: Polyamines during somatic embryo development in Norway spruce (Picea abies [L.]). | JOURNAL OF FOREST SCIENCE 55 2009 2 75-80 Polyamines during somatic embryo development in Norway spruce Picea abies L. J. Malá1 M. Cvikrová2 P. Máchová2 O. Martincová2 1Forestry and Game Management Research Institute Strnady Czech Republic Institute of Experimental Botany Academy of Sciences of the Czech Republic Prague Czech Republic ABSTRACT Contents of free polyamines putrescine spermidine and spermine were determined in different developmental stages of Norway spruce Picea abies L. Karst. somatic embryos by means of HPLC. Determinations were performed embryogenic tissue after 4 weeks of the growth on proliferation medium after 2 and 5 weeks of the culturing on maturation medium and 2 weeks after desiccation. Maturation of somatic embryos after 5 weeks was accompanied by increase of concentrations of putrescine 2.3 times and spermidine 3.2 times . In comparison with above mentioned polyamines spermine concentrations were significantly lower 4.3 times . Two weeks after desiccation the concentrations of putrescine decreased 5.4 times and spermidine 2.2 times in comparison with mature embryos. To improve the efficiency of somatic embryogenesis of less responsive genotypes the supplementation of growth media by polyamines is discussed. Keywords Norway spruce somatic embryogenesis putrescine spermidine spermine Abbreviations abscisic acid - ABA 6-benzylaminopurine - BAP cytokinins - CKs 2 4 dichlorophenoxyacetic acid - 2 4-D indolylbutyric acid - IBA putrescine - Put spermidine - Spm spermine - Spd Somatic embryogenesis is considered as an advantageous technique for in vitro propagation of conifers. Moreover it could be usefully utilized for a detailed study of developmental processes accompanying differentiation of embryo and its conversion during polyembryogenesis. Regeneration of complete plants by means of somatic embryogenesis in the Norway spruce Picea abies L. Karst. was described by many authors Boenman 1985 Hakman et al. 1985 Attree Fowke 1993 Chalupa 1985 .

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