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Báo cáo khoa học: "Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD | J. Vet. Sci. 2005 6 1 7-19 JOURNAL OF Veterinary Science Isolation and identification of Escherichia coli O157 H7 using different detection methods and molecular determination by multiplex PCR and RAPD Ji-Yeon Kim1 2 So-Hyun Kim2 Nam-Hoon Kwon2 Won-Ki Bae2 Ji-Youn Lim2 Hye-Cheong Koo2 Jun-Man Kim2 Kyoung-Min Noh2 Woo-Kyung Jung2 Kun-Taek Park2 Yong-Ho Park2 1 Department of Animal Disease Diagnosis National Veterinary Research and Quarantine Service Anyang 430-824 Korea 2Department of Microbiology College of Veterinary Medicine and School of Agricultural Biotechnology Seoul National University Seoul 151-742 Korea Escherichia coli O157 H7 is recognized as a significant food-borne pathogen so rapid identification is important for food hygiene management and prompt epidemiological investigations. The limited prevalence data on Shiga toxinproducing E. coli STEC and E. coli O157 H7 in foods and animals in Korea made an assessment of the risks difficult and the options for management and control unclear. The prevalence of the organisms was examined by newly developed kit-E. coli O157 H7 Rapid kit. For the isolation of E. coli O157 H7 conventional culture immunomagnetic separation and E. coli O157 H7 Rapid kit were applied and multiplex PCR and randomly amplified polymorphic DNA RAPD were performed for the molecular determination. There was high molecular relatedness among 11 Korean isolates and 17 U. S. strains at 63 level. Additionally distinct differentiation between pig and cattle isolates was determined. It implied that RAPD had a capacity to distinguish strains with different sources however it could not discriminate among isolates according to their differences in the degree of virulence. In antimicrobial susceptibility tests 45.5 of isolates showed antibiotic resistance to two or more antibiotics. Unlike the isolates from other countries domestic isolates of E. coli O157 H7 was mainly resistant to ampicillin and tetracylines. In summary the application of E. coli .