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Báo cáo khoa học: "Molecular cloning of the cDNA of canine homeodomaininteracting protein kinase 2"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Molecular cloning of the cDNA of canine homeodomaininteracting protein kinase 2 | J. Vet. Sci. 2005 6 2 141-145 JOURNAL OF Veterinary Science Molecular cloning of the cDNA of canine homeodomain-interacting protein kinase 2 Sook-Yeon Lee Jin-Young Chung Il-Seob Shin Eun-Wha Choi Cheol-Yong Hwang Hwa-Young Youn Hong-Ryul Han Department of Veterinary Internal Medicine College of Veterinary Medicine and School of Agricultural Biotechnology Seoul National University Seoul 151-742 Korea The research of p53 is being conducted to find the mechanisms of tumorigenesis and to treat various cancers. Homeodomain-interacting protein kinase2 HIPK2 is an important factor to regulate p53 and to increase the stability of p53. Activation of HIPK2 leads to the selective phosphorylation of p53 resulting in growth arrest and the enhancement of apoptosis. In this study the canine HIPK2 cDNA fragments were obtained and their overlapping regions were aligned to give a total sequence of 3489 bp. The canine HIPK2 cDNA GenBank accession number AY800385 shares 93 and 90 sequence identity with those of human and mouse HIPK2 respectively. The canine HIPK2 cDNA contains an open reading frame encoding 1163 amino acid residues and the predicted amino acid sequence has 98 and 96 identity with those of human and mouse respectively. The deduced amino acid sequence of canine HIPK2 has also all domains sites compared with human and mouse HIPK2. Therefore these structural similarities suggested that the canine HIPK2 shares the basic biological functions that HIPK2 exhibit in other species. Key words cloning dog HIPK2 p53 regulation Introduction Homeodomain-interacting protein kinases HIPKs constitute a novel family of nuclear protein kinases. Three members of this family HIPK1 HIPK2 and HIPK3 have been isolated in human and mouse so far but none of those was isolated in dogs. HIPK2 has been described as a homeodomain-interacting protein kinase which acts as a co-repressor for homeodomain transcription factors 10 . HIPK2 colocalizes with p53 in nuclear bodies and phosphorylates p53. .