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Báo cáo khoa học: " Modification of maturation condition improves oocyte maturation and in vitro development of somatic cell nuclear transfer pig embryos"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Modification of maturation condition improves oocyte maturation and in vitro development of somatic cell nuclear transfer pig embryos | J. Vet. Set. 2007 8 1 81-87 JOURNAL OF Veterinary Science Modification of maturation condition improves oocyte maturation and in vitro development of somatic cell nuclear transfer pig embryos Kilyoung Song1 Eunsong Lee2 1 College of Veterinary Medicine Seoul National University Seoul 151-742 Korea 2School of Veterinary Medicine and Institute of Veterinary Science Kangwon National University Chunchon 200-701 Korea This study examined effects on the developmental competence of pig oocytes after somatic cell nuclear transfer SCNT or parthenogenetic activation PA of 1 co-culturing of oocytes with follicular shell pieces FSP during in vitro maturation IVM 2 different durations of maturation and 3 defined maturation medium supplemented with polyvinyl alcohol PVA control pig follicular fluid pFF cysteamine CYS or P-mercapto-ethanol P-ME . The proportion of metaphase II oocytes was increased p 0.05 by co-culturing with FSP compared to control oocytes 98 vs. 94 . However blastocyst formation after SCNT was not improved by FSP coculture 9 vs. 12 . Nuclear maturation of oocytes matured for 39 or 42 h was higher p 0.05 than that of oocytes matured for 36 h 95-96 vs. 79 . Cleavage 83 and blastocyst formation 26 were significantly higher p 0.05 in oocytes matured for 42 h than in other groups. Supplementation of a defined maturation medium with 100 LiM CYS or 100 LiM P-ME showed no stimulatory effect on oocyte maturation embryo cleavage or blastocyst formation after PA. P-ME treatment during IVM decreased embryo cleavage after SCNT compared to pFF or PVA treatments but no significant difference was found in blastocyst formation 7-16 among the four treatment groups. The results indicated that maturation of oocytes for 42 h was beneficial for the development of SCNT embryos. Furthermore the defined maturation system used in this study could support in vitro development of PA or SC NT embryos. Key words embryo development oocyte maturation parthenogenesis pig somatic cell nuclear .