Đang chuẩn bị liên kết để tải về tài liệu:
Báo cáo khoa học: "Comparison of the age-related porcine endogenous retrovirus (PERV) expression using duplex RT-PCR"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Comparison of the age-related porcine endogenous retrovirus (PERV) expression using duplex RT-PCR | J. Vet. Sci. 2009 10 4 317-322 DOI 10.4142 jvs.2009.10.4.317 JOURNAL OF Veterinary Science Comparison of the age-related porcine endogenous retrovirus PERV expression using duplex RT-PCR Hyoung Joon Moon Hye Kwon Kim Seong Jun Park Chul Seung Lee Dae Sub Song Bo Kyu Kang Bong Kyun Park Department of Veterinary Medicine Virology Lab College of Veterinary Medicine and BK21 Program for Veterinary Science Seoul National University Seoul 151-742 Korea Porcine endogenous retroviruses PERVs are members of family Retroviridae genus Gamma retrovirus and transmitted by both horizontally and vertically like other endogenous retroviruses ERVs . PERV was initially described in the 1970s having inserted its gene in the host genome of different pig breeds and three classes PERV-A PERV-B and PERV-C are known. The therapeutic use of living cells tissues and organs from animals called xenotransplantation might relieve the limited supply of allografts in the treatment of organ dysfunction. Because of ethical considerations compatible organ sizes and physiology the pig has been regarded as an alternative source for xenotransplantation. Sensitive duplex reverse transcription-polymerase chain reaction protocols for simultaneously detecting PERV gag mRNA and porcine glyceraldehydes 3-phosphate dehydrogenase mRNA in one tube was established. To compare the age-related PERV expression patterns of the lung liver spleen kidney heart and pancreas in commercial pigs 20 pigs from four age groups 5 heads each in 10 days- 40 days- 70 days- and 110 days-old respectively were used in this study. The expression patterns of PERV were statistically different among age groups in lung liver and kidney ANOVA p 0.05 . These data may support in the selection of appropriate donor pigs expressing low levels of PERV mRNA. Keywords mRNA expression PERV pig RT-PCR Introduction Endogenous retroviruses ERVs are integrated into the germ-line of vertebrates and transmitted to their offspring by Mendelian genetics.