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Báo cáo khoa học: "Towards construction of an ultra high density linkage map for Pinus pinaster"

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp quốc tế đề tài: Towards construction of an ultra high density linkage map for Pinus pinaster. | Ann. For. Sci. 59 2002 637-643 INRA EDP Sciences 2002 DOI 10.1051 forest 2002049 637 Original article Towards construction of an ultra high density linkage map for Pinus pinaster I TV T T Z 111ÍA 1 -ỉ t-ÝíAT aA TV o Ạ T o Cĩ AĨÌ Ốoa l A t orlyi IO CỠHb ìrnỉ A rvlv i v c QJ o TV Ý1 o í Tf I 0 TAI TV ZaI a Enrique Ritter Ana Aragonés Torsten Markussen Virginie Acheré Santiago Espinel Matthias Fladungb Sandra Wrobelb Patricia Faivre-Rampantc Sylvain Jeandrozc and Jean-Michel Favrec aNEIKER Apartado 46 Vitoria Alava 01080 Spain bBFH Institute for Forest Genetics Sieker Landstrasse 2 Grosshansdorf 22927 Germany cUMR UHP-INRA Plant-Microbes Interactions Faculte des Sciences BP 239 54506 Vandreuvre-lès-Nancy France Received 16 August 2001 accepted 22 February 2002 Abstract- Two parental linkage maps have been constructed from the P. pinaster reference population 0024 X C803 based on AFLP SSR and EST markers. Although segregating polymorphism was low due to a high degree of homozygosity in the parents 12 linkage groups with 26 to 46 markers each were obtained for each parent. The availibility of 70 anchor points based on fragments common to both parents and based on codominant SSR and EST markers allowed to determine homologous chromosomes for both maps and to construct one integrated map. Total genome length of the integrated map is around 2000 cM including 1182 markers. Since some of the EST and SSR markers are also mapped in different pine species association of linkage groups of our reference population with those of other published maps was possible. AFLP SSR EST markers genetic mapping Résumé - Vers la construction d une carte génétique ultra-haute densité chez Pinus pinaster. Deux cartes génétiques ont été construites à partird un croisement intra-spécifique de P. Pinaster 0024 Landes X C803 Corse avec des marqueurs AFLP SSR et EST. Malgréun faiblepo-lymorphisme du à la forte homozygotie des parents 12 groupes de liaison comprenant 26 à 46 marqueurs ont été obtenus