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Báo cáo y học: "Pravastatin Provides Antioxidant Activity and Protection of Erythrocytes Loaded Primaqe"

Tuyển tập các báo cáo nghiên cứu khoa học ngành y học tạp chí Medical Sciences dành cho các bạn sinh viên ngành y tham khảo đề tài: Pravastatin Provides Antioxidant Activity and Protection of Erythrocytes Loaded Primaquine | Int. J. Med. Sci. 2010 7 358 International Journal of Medical Sciences 2010 7 6 358-365 Ivyspring International Publisher. All rights reserved Research Paper Pravastatin Provides Antioxidant Activity and Protection of Erythrocytes Loaded Primaquine Fars K. Alanazi 1 2 H 1. Kayyali Chair for Pharmaceutical Industry Department of Pharmaceutics College of Pharmacy King Saud University P.O. Box 2457 Riyadh 11451 Saudi Arabia. 2. Center of Excellence in Biotechnology Research King Saud University P.O. Box 2460 Riyadh 11451 Saudi Arabia. H Corresponding author Email afars@yahoo.com. Received 2010.09.02 Accepted 2010.10.27 Published 2010.10.28 Abstract Loading erythrocytes with Primaquine PQ is advantageous. However PQ produces damage to erythrocytes through free radicals production. Statins have antioxidant action and are involved in protective effect against situation of oxidative stress. Thus the protective effect of pravastatin PS against PQ induced oxidative damage to human erythrocytes was investigated in the current studies upon loading to erythrocytes. The erythrocytes were classified into control erythrocytes erythrocytes incubated with either 2 mM of PS or 2 mM of PQ and erythrocytes incubated with combination of PS plus PQ. After incubation for 30 min the effect of the drugs on erythrocytes hemolysis as well as some biomarkers of oxidative stress none protein thiols protein carbonyl thiobarbituric acid reactive substance were investigated. Our results revealed that PS maintains these biomarkers at values similar to that of control ones. On the other hand PQ cause significant increases of protein carbonyl by 115 and thiobarbituric acid reactive substance by 225 while non-protein thiols were significantly decreased by 112 compared with control erythrocytes. PS pre-incubation before PQ exerts marked reduction of these markers in comparison with PQ alone. Moreover at NaCl concentrations between 0.4 and 0.8 PQ causes significant increase of Red Blood Cells RBCs .