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Báo cáo y học: "TSLP directly impairs pulmonary Treg function: association with aberrant tolerogenic immunity in asthmatic airway"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: TSLP directly impairs pulmonary Treg function: association with aberrant tolerogenic immunity in asthmatic airway. | Nguyen et al. Allergy Asthma Clinical Immunology 2010 6 4 http www.aacijournal.eom content 6 1 4 ALLERGY ASTHMA CLINICAL IMMUNOLOGY RESEARCH Open Access TSLP directly impairs pulmonary Treg function association with aberrant tolerogenic immunity in asthmatic airway Khoa D Nguyen Christopher Vanichsarn Kari C Nadeau Abstract Background Even though thymic stromal lymphopoietin TSLP has been implicated in the development of allergic inflammation its influence on immune tolerance mediated by regulatory T cells Treg have not been explored. We aimed to dissect the influence of TSLP on immunosuppressive activities of Treg and its potential consequences in human allergic asthma. Methods In vitro culture system was utilized to study the effects of TSLP on human Treg. The functional competency of pulmonary Treg from a cohort of 15 allergic asthmatic 15 healthy control and 15 non-allergic asthmatic subjects was also evaluated by suppression assays and flow cytometric analysis. Results Activated pulmonary Treg expressed TSLP-R and responded to TSLP-mediated activation of STAT5. TSLP directly and selectively impaired IL-10 production of Treg and inhibited their suppressive activity. In human allergic asthma pulmonary Treg exhibited a significant decrease in suppressive activity and IL-10 production compared to healthy control and non-allergic asthmatic counterparts. These functional alterations were associated with elevated TSLP expression in bronchoaveolar lavage fluid BAL of allergic asthmatic subjects. Furthermore allergic asthmatic BAL could suppress IL-10 production by healthy control pulmonary Treg in a TSLP-dependent manner. Conclusions These results provide the first evidences for a direct role ofTSLP in the regulation of suppressive activities of Treg. TSLP mediated inhibition of Treg function might present a novel pathologic mechanism to dampen tolerogenic immune responses in inflamed asthmatic airway. Background Thymic stromal lymphopoietin TSLP was initially .