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Báo cáo y học: "The active metabolite of leflunomide, A77 1726, increases the production of IL-1 receptor antagonist in human synovial fibroblasts and articular chondrocytes"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: The active metabolite of leflunomide, A77 1726, increases the production of IL-1 receptor antagonist in human synovial fibroblasts and articular chondrocytes. | Available online http arthritis-research.eom content 6 3 R181 Research article Open Access The active metabolite of leflunomide A77 1726 increases the production of IL-1 receptor antagonist in human synovial fibroblasts and articular chondrocytes Gaby Palmer1 Danielle Burger2 Frangoise Mezin1 David Magne1 Cem Gabay1 Jean-Michel Dayer2 and Pierre-André Guerne1 1 Division of Rheumatology University Hospital and Department of Pathology University of Geneva School of Medicine Geneva Switzerland 2Division of Immunology and Allergy Department of Internal Medicine University Hospital Geneva Switzerland Corresponding author Pierre-André Guerne e-mail Pierre-Andre.Guerne@medecine.unige.ch Received 12 Sep 2003 Revisions requested 10 Oct 2003 Revisions received 27 Jan 2004 Accepted 4 Feb 2004 Published 19 Feb 2004 Arthritis Res Ther 2004 6 R181-R189 DOI 10.1186 ar1157 2004 Palmer et al. licensee BioMed Central Ltd. This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Abstract Leflunomide is an immunomodulatory agent used for the treatment of rheumatoid arthritis. In this study we investigated the effect of A77 1726 - the active metabolite of leflunomide -on the production of IL-1 receptor antagonist IL-1Ra by human synovial fibroblasts and articular chondrocytes. Cells were incubated with A77 1726 alone or in combination with proinflammatory cytokines. IL-1Ra production was determined by ELISA. A77 1726 alone had no effect but in the presence of IL-1P or tumour necrosis factor-a it markedly enhanced the secretion of IL-1Ra in synovial fibroblasts and chondrocytes. The effect of A77 1726 was greatest at 100 pmol l. In synovial fibroblasts and de-differentiated chondrocytes A77 1726 also increased IL-1 p-induced IL-1Ra production in cell lysates. Freshly isolated chondrocytes contained no significant amounts of intracellular IL-1Ra. .