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Báo cáo lâm nghiệp: " An for attempt at generating haploid lines of Poplar species genetic manipulation and breeding program"

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp Original article đề tài: An for attempt at generating haploid lines of Poplar species genetic manipulation and breeding program. | 101s Ann. Sei. For. 1989 46 suppl. 101s-107s Forest Tree Physiology E. Dreyer et al. eds. Elsevier INRA Physiological aspects of somatic polyembryogenesis in suspension cultures of conifers D.J. Durzan Department of Environmental Horticulture University of California Davis CA 95616 U.S.A. Introduction Somatic polyembryogenesis SPE is a new cell culture technology that needs to be distinguished from somatic embryogenesis and other forms of regeneration cf. Durzan 1988a . SPE involving the reconstitution of multiple embryos by cleavage or budding of a proembryo is one of 3 categories of regeneration recognized by Sinnott 1960 . It is one of 4 broad categories of polyembryogenesis see Table I . Process control of SPE in cell suspension cultures for the multiplication of tree crops has been reviewed by Durzan 1988a b and Durzan and Gupta 1988 . Using cell suspension cultures of embryonal-suspensor masses ESMs from loblolly pine and Douglas fir morpho-genic protoplasts have been prepared that enable the recovery of somatic embryos and the transient expression of a foreign gene uc Gupta and Durzan 1987a Gupta et al. 1988 . Somatic embryos have also been recovered from cryopreserved ESMs Gupta et al. 1987 . In this review several recently recognized phy- Table I. Products produced by polyembryogenesis as a form of agamospermy Durzan 1988a . Type Product attributes Simple Several zygotes but one embryo from one zygote to produce groups of embryos of fraternal type not clones Cleavage and budding Asexual reconstitution of transplanted zygotic embryos embryonal-suspensor masses yielding an identical type clones . One or more groups of a given fraternal type are possible. Cleavage and budding are similar except in terms of source and topological orientation of the reconstituted embryos. Sporophytic Adventitious embryos arise by budding from the nucellus clones of the sporophyte or from differentiated embryonal cells clones of the new generation . Gametophytic The haploid .