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Báo cáo y học: "VLA-4-dependent and -independent pathways in cell contactinduced proinflammatory cytokine production by synovial nurselike cells from rheumatoid arthritis patients"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: VLA-4-dependent and -independent pathways in cell contactinduced proinflammatory cytokine production by synovial nurselike cells from rheumatoid arthritis patients. | Available online http arthritis-research.eom 4 6 R10 Research article VLA-4-dependent and -independent pathways in cell contact-induced proinflammatory cytokine production by synovial nurselike cells from rheumatoid arthritis patients Eiji Takeuchi1 2 4 Toshiyuki Tanaka1 Eiji Umemoto1 Tetsuya Tomita2 Kenrin Shi2 Koichiro Takahi2 Ryuji Suzuki3 Takahiro Ochi2 and Masayuki Miyasaka1 Laboratory of Molecular and Cellular Recognition Osaka University Graduate School of Medicine Osaka Japan 2Department of Orthopaedic Surgery Osaka University Graduate School of Medicine Osaka Japan 3Research Unit of Immunology Shionogi Institute for Medical Science Shionogi Co. Ltd Osaka Japan current address Pharmacology Research Laboratories Pharmaceutical Research Division Takeda Chemical Industries Ltd Osaka Japan 4Current address Department of Orthopaedic Surgery Osaka Rousai Hospital Osaka Japan Correspondence Masayuki Miyasaka e-mail mmiyasak@orgctl.med.osaka-u.ac.jp Received 26 June 2002 Revisions received 4 July 2002 Accepted 15 July 2002 Published 12 August 2002 Arthritis Res 2002 4 R10 DOI 10.1186 ar593 2002 Takeuchi et al. licensee BioMed Central Ltd Print ISSN 1465-9905 Online ISSN 1465-9913 Abstract Nurse-like stromal cell lines from the synovial tissue of patients with rheumatoid arthritis RA-SNC produce on coculture with lymphocytes large amounts of proinflammatory cytokines. In the present paper we analyze the molecular events necessary for the induction of cytokine release from RA-SNC cells and particularly the roles played by cell adhesion and the transmigration also known as pseudoemperipolesis of lymphocytes. For this purpose the effects of various mAbs on the binding and transmigration of a human B-cell line MC car were examined using a cloned RA-SNC line RA-SNC77. To analyze the role of lymphocyte binding and transmigration on upregulated cytokine production by the RA-SNC77 cells we used C3 exoenzyme-treated MC car cells which could bind to RA-SNC77 cells but could .