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Báo cáo y học: "Chondrocytes, synoviocytes and dermal fibroblasts all express PH-20, a hyaluronidase active at neutral pH"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Chondrocytes, synoviocytes and dermal fibroblasts all express PH-20, a hyaluronidase active at neutral pH. | Available online http arthritis-research.eom content 7 4 R756 Research article Chondrocytes synoviocytes and dermal fibroblasts all express PH-20 a hyaluronidase active at neutral pH Hafida El Hajjaji1 Ada Asbury Cole2 and Daniel-Henri Manicourt1 3 Christian de Duve Institute of Cellular Pathology Department of Biochemistry Connective Tissue Group Université Catholique de Louvain in Brussels Brussels Belgium 2Department of Biochemistry Rush Medical College Rush-Presbyterian-St. Luke s Medical Center Chicago IL USA 3Department of Rheumatology Saint Luke s University Hospital Catholic University of Louvain in Brussels Brussels Belgium Corresponding author Daniel-Henri Manicourt manicourt@bchm.ucl.ac.be Received 18 Mar 2004 Revisions requested 8 Apr 2004 Revisions received 21 Feb 2005 Accepted 7 Mar 2005 Published 4 Apr 2005 Arthritis Research Therapy 2005 7 R756-R768 DOI 10.11 86 ar1 730 This article is online at http arthritis-research.com content 7 4 R756 2005 Hajjaji et al. licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract Hyaluronan HA an important component of connective tissues is highly metabolically active but the mechanisms involved in its catabolism are still largely unknown. We hypothesized that a protein similar to sperm PH-20 the only mammalian hyaluronidase known to be active at neutral pH could be expressed in connective tissue cells. An mRNA transcript similar to that of PH-20 was found in chondrocytes synoviocytes and dermal fibroblasts and its levels were enhanced upon stimulation with IL-1. In cell layers extracted with Triton X-100 - but not with octylglucoside - and in culture media a polyclonal antipeptide anti-PH-20 antibody identified protein bands with a molecular weight similar to