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Báo cáo y học: "The induction of CCN2 by TGFβ1 involves Ets-1"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: The induction of CCN2 by TGFβ1 involves Ets-1. | Available online http arthritis-research.eom content 8 2 R36 Research article The induction of CCN2 by TGFp1 involves Ets-1 Jonathan P Van Beek Laura Kennedy Jason S Rockel Suzanne M Bernier and Andrew Leask CIHR Group in Skeletal Development and Remodeling Schulich School of Medicine and Dentistry Dental Sciences Building The University of Western Ontario London ON N6A 5C1 Canada Corresponding author Andrew Leask Andrew.Leask@schulich.uwo.ca Received 15 Oct 2005 Revisions requested 15 Dec 2005 Revisions received 19 Dec 2005 Accepted 19 Dec 2005 Published 16 Jan 2006 Arthritis Research Therapy 2006 8 R36 doi 10.1186 ar1 890 This article is online at http arthritis-research.com content 8 2 R36 2006 Van Beek et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract CCN2 is encoded by an immediate-early gene induced in mesenchymal cells during the formation of blood vessels bone and connective tissue. It plays key roles in cell adhesion and migration as well as matrix remodeling. CCN2 is overexpressed in fibrosis arthritis and cancer thus an understanding of how to control CCN2 expression is likely to have importance in developing therapies to combat these pathologies. Previously we found that the promoter sequence GAGGAATG is important for Ccn2 gene regulation in NIH 3T3 fibroblasts. In this report we show that this sequence mediates activation of the CCN2 promoter by the ETS family of transcription factors. Endogenous Ets-1 binds this element of the CCN2 promoter and dominant negative Ets-1 and specific Ets-1 small interfering RNA block induction of CCN2 expression by TGFp. In the absence of added TGFP1 Ets-1 but not the related fli-1 synergizes with Smad 3 to activate the CCN2 promoter. Whereas the ability