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Báo cáo y học: "Is disturbed clearance of apoptotic keratinocytes responsible for UVB-induced inflammatory skin lesions in systemic lupus erythematosus"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Is disturbed clearance of apoptotic keratinocytes responsible for UVB-induced inflammatory skin lesions in systemic lupus erythematosus. | Available online http arthritis-research.eom content 8 6 R157 Research article PUMA-mediated apoptosis in fibroblast-like synoviocytes does not require p53 Xin You David L Boyle Deepa Hammaker and Gary S Firestein Division of Rheumatology Allergy and Immunology University of California at San Diego School of Medicine 9500 Gilman Drive La Jolla California 92093 USA Corresponding author Gary S Firestein gfirestein@ucsd.edu Received 5 Jul 2006 Revisions requested 14 Aug 2006 Revisions received 13 Sep 2006 Accepted 2 Oct 2006 Published 2 Oct 2006 Arthritis Research Therapy 2006 8 R157 doi 10.1186 ar2052 This article is online at http arthritis-research.com content 8 6 R157 2006 You et al licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract PUMA p53-upregulated modulator of apoptosis is a pro-apoptotic gene that can induce rapid cell death through a p53-dependent mechanism. However the efficacy of PUMA gene therapy to induce synovial apoptosis in rheumatoid arthritis might have limited efficacy if p53 expression or function is deficient. To evaluate this issue studies were performed to determine whether p53 is required for PUMA-mediated apoptosis in fibroblast-like synoviocytes FLS . p53 protein was depleted or inhibited in human FLS by using p53 siRNA or a dominant-negative p53 protein. Wild-type and p53 _ murine FLS were also examined to evaluate whether p53 is required. p53-deficient or control FLS were transfected with PUMA cDNA or empty vector. p53 and p21 expression were then determined by Western blot analysis. Apoptosis was assayed by ELISA to measure histone release and caspase-3 activation or by trypan blue dye exclusion to measure cell viability. Initial studies showed that p53 siRNA decreased p53 .