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Báo cáo y học: "Analysis of normal and osteoarthritic canine cartilage mRNA expression by quantitative polymerase chain reacti"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Analysis of normal and osteoarthritic canine cartilage mRNA expression by quantitative polymerase chain reaction. | Available online http arthritis-research.eom content 8 6 R158 Research article Analysis of normal and osteoarthritic canine cartilage mRNA expression by quantitative polymerase chain reaction Dylan N Clements1 2 Stuart D Carter1 John F Innes1 William ER Ollier2 and Philip JR Day2 1The Musculoskeletal Research Group c o Department of Veterinary Pathology Faculty of Veterinary Science University of Liverpool Liverpool L69 3BX UK __ _ 2Centre for Integrated Genomic Medical Research The Stopford Building University of Manchester Oxford Road Manchester M13 9PT UK Corresponding author Dylan N Clements dylan.clements@liverpool.ac.uk Received 26 Apr 2006 Revisions requested 18 May 2006 Revisions received 7 Aug 2006 Accepted 10 Oct 2006 Published 10 Oct 2006 Arthritis Research Therapy 2006 8 R158 doi 10.1186 ar2053 This article is online at http arthritis-research.com content 8 6 R158 2006 Clements et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract The molecular basis to mammalian osteoarthritis OA is unknown. We hypothesised that the expression of selected proteases matrix molecules and collagens believed to have a role in the pathogenesis of OA would be changed in naturally occurring canine OA cartilage when compared to normal articular cartilage. Quantitative real-time reverse transcriptase-polymerase chain reaction assays were designed measuring the expression of selected matrix molecules collagens and small leucine-rich proteoglycans key mediators of the proteolytic degradation of articular cartilage metalloproteinases cathepsins and their inhibitors tissue inhibitors of matrix metalloproteinases . All data were normalised using a geometric mean of three housekeeping genes and the results subjected .