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Báo cáo khoa học: "High throughput evaluation of gamma-H2AX Dane Avondoglio1, Tamalee Scott1, Whoon Jong Kil, Mary "

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Radiation Oncology cung cấp cho các bạn kiến thức về ngành y đề tài: "High throughput evaluation of gamma-H2AX Dane Avondoglio1, Tamalee Scott1, Whoon Jong Kil, Mary. | Radiation Oncology BioMed Central Open Access High throughput evaluation of gamma-H2AX Dane Avondoglio1 Tamalee Scott1 Whoon Jong Kil Mary Sproull1 Philip J Tofilon2 and Kevin Camphausen 1 Address Radiation Oncology Branch National Cancer Institute National Cancer Institute Bethesda Maryland USA and 2Drug Discovery Program H. Lee Moffitt Cancer Center Tampa Florida USA Email Dane Avondoglio - avondogd@mail.nih.gov Tamalee Scott - scottta@mail.nih.gov Whoon Jong Kil - kilwh@mail.nih.gov Mary Sproull - sproullm@mail.nih.gov Philip J Tofilon - philip.tofilon@moffitt.org Kevin Camphausen - camphauk@mail.nih.gov Corresponding author Published 24 August 2009 Received 2 June 2009 Accepted 24 August 2009 Radiation Oncology 2009 4 3 1 doi 10.1186 1748-717X-4-31 This article is available from http www.ro-journal.cOm content 4 1 31 2009 Avondoglio et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract The DNA double-strand break DSB is the primary lethal lesion after therapeutic radiation. Thus the development of assays to detect and to quantitate these lesions could have broad preclinical and clinical impact. Phosphorylation of histone H2AX to form Y-H2AX is a known marker for irradiation-induced DNA DSBs. However the first generation assay involves the use of immunofluorescent staining of Y-H2AX foci. This assay is time consuming operator dependent and is not scalable for high throughput assay development. Thus we sought to develop a new assay using a high throughput electrochemiluminescent platform from Mesoscale Discovery Systems to quantify Y-H2AX levels. The results show that our assay utilizes significantly less time and labor has greater intra-assay reproducibility and has a greater dynamic range of Y-H2AX versus .