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Báo cáo y học: "Glucosamine affects intracellular signalling through inhibition of mitogen-activated protein kinase phosphorylation in human chondrocytes"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Glucosamine affects intracellular signalling through inhibition of mitogen-activated protein kinase phosphorylation in human chondrocytes. | Available online http arthritis-research.eom content 9 5 R104 Research article Glucosamine affects intracellular signalling through inhibition of mitogen-activated protein kinase phosphorylation in human chondrocytes Anna Scotto d Abusco1 Valentina Calamia1 Claudia Cicione1 Brunella Grigolo2 Laura Politi1 and Roberto Scandurra1 1 Department of Biochemical Sciences Sapienza University of Roma P.le Aldo Moro 5 00185 Roma Italy laboratory of Immunology and Genetics Istituto di Ricerca Codivilla Putti Istituti Ortopedici Rizzoli Via di Barbiano 1 10 40136 Bologna Italy Corresponding author Anna Scotto d Abusco anna.scottodabusco@uniroma1 .it Received 9 May 2007 Revisions requested 22 Jun 2007 Revisions received 1 7 Sep 2007 Accepted 9 Oct 2007 Published 9 Oct 2007 Arthritis Research Therapy 2007 9 R104 doi 10.1186 ar2307 This article is online at http arthritis-research.com content 9 5 R104 2007 Scotto d Abusco et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract The aim of this study was to determine the effects of glucosamine on matrix metalloprotease MMP production on mitogen-activated protein kinase MAPK phosphorylation and on activator protein AP -1 transcription factor activation in human chondrocytes. The human immortalized cell line lbpva55 and healthy human chondrocytes obtained from healthy donors were subjected to challenge with 10 ng ml IL-1P after pretreatment with 2.5 or 10 mmol l glucosamine. MMP mRNA expression levels were evaluated using quantitative real-time PCR and MMP protein production levels were evaluated in the culture supernatant using ELISA. MAPK phosphorylation was evaluated using Western blotting. AP-1 transcription factor activation was evaluated by measuring AP-1 DNA-binding