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Báo cáo y học: "Diacerein inhibits the synthesis of resorptive enzymes and reduces osteoclastic differentiation/survival in osteoarthritic subchondral bone: a possible mechanism for a protective effect against subchondral bone remodelling"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Diacerein inhibits the synthesis of resorptive enzymes and reduces osteoclastic differentiation/survival in osteoarthritic subchondral bone: a possible mechanism for a protective effect against subchondral bone remodelling. | Available online http arthritis-research.eom content 10 3 R71 Research article Diacerein inhibits the synthesis of resorptive enzymes and reduces osteoclastic differentiation survival in osteoarthritic subchondral bone a possible mechanism for a protective effect against subchondral bone remodelling Christelle Boileau Steeve Kwan Tat Jean-Pierre Pelletier Saranette Cheng and Johanne Martel-Pelletier Osteoarthritis Research Unit University of Montreal Hospital Centre Notre-Dame Hospital 1560 Sherbrooke Street East Montreal Quebec H2L 4M1 Canada Corresponding author Johanne Martel-Pelletier jm@martelpelletier.ca Received 9 Apr 2008 Revisions requested 29 May 2008 Revisions received 5 Jun 2008 Accepted 25 Jun 2008 Published 25 Jun 2008 Arthritis Research Therapy 2008 10 R71 doi 10.1186 ar2444 This article is online at http arthritis-research.com content 10 3 R71 2008 Boileau et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract Introduction Subchondral bone alterations represent an essential component of osteoarthritis OA . Modifying the abnormal subchondral bone metabolism may be indicated to treat OA. We investigated the effect of diacerein and rhein on the changes occurring in subchondral bone during OA. To this end we determined the drugs effects on metalloprotease-13 MMP-13 synthesis on subchondral bone and on the osteoblast signalling pathways. In osteoclasts we studied MMP-13 and cathepsin K production as well as cell differentiation proliferation and survival. Methods The effect of diacerein rhein on the production of subchondral bone MMP-13 was determined by enzyme-linked immunosorbent assay. Signalling pathways were evaluated on osteoblasts by Western blot. Osteoclast experiments were performed