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Báo cáo y học: "Limited reliability of the indirect immunofluorescence technique for the detection of anti-Rib-P antibodies"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Limited reliability of the indirect immunofluorescence technique for the detection of anti-Rib-P antibodies. | Available online http arthritis-research.eom content 10 6 R131 Open Access Research article Limited reliability of the indirect immunofluorescence technique for the detection of anti-Rib-P antibodies Michael Mahler1 Jennifer T Ngo2 Johannes Schulte-Pelkum1 Tanja Luettich3 and Marvin J Fritzler2 1Dr. Fooke Laboratorien GmbH MainstraBe 85 D-41469 Neuss Germany 2Department of Biochemistry and Molecular Biology University of Calgary 3330 Hospital Drive NW Calgary AB T2N 4N1 Canada 3Mikrogen GmbH Floriansbogen 2-4 82061 Neuried Germany Corresponding author Marvin J Fritzler fritzler@ucalgary.ca Received 5 Aug 2008 Revisions requested 19 Sep 2008 Revisions received 26 Oct 2008 Accepted 11 Nov 2008 Published 11 Nov 2008 Arthritis Research Therapy 2008 10 R131 doi 10.11 86 ar2548 This article is online at http arthritis-research.com content 10 6 R131 2008 Mahler et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Introduction Autoantibodies to the ribosomal P proteins represent a highly specific marker for the diagnosis of systemic lupus erythematosus where they have been associated with certain clinical manifestations. Historically autoantibodies against ribosomal P proteins have been detected by indirect immunofluorescence immunodiffusion immunoblot and other immunoassays. More recently enzyme-linked immunosorbent assays and line and addressable laser bead immunoassays have become more widely used. The primary goal of this study was to determine the sensitivity of indirect immunofluorescence using conventional HEp-2 substrates in the detection of sera with ribosomal P antibodies as detected by other immunoassays. Methods Anti-ribosomal P-positive sera n 345 as detected by an addressable laser bead immunoassay were .