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Báo cáo y học: " Hypertonic saline increases lung epithelial lining fluid glutathione and thiocyanate: two protective "

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:Hypertonic saline increases lung epithelial lining fluid glutathione and thiocyanate: two protective . | Gould et al. Respiratory Research 2010 11 119 http respiratory-research.eom content 11 1 119 RESPIRATORY RESEARCH RESEARCH Open Access Hypertonic saline increases lung epithelial lining fluid glutathione and thiocyanate two protective CFTR-dependent thiols against oxidative injury I I c r _ I 11 -1 3 4 c r _ I if-1 I r4 I i r ị I lz 3 4 1 I I 4 I I I_I I n r Á 4 DZ n I I ì Ỉ 1 2 3 4 5 Neal S Gould Steve Gauthier Chirag I Kariya Elysia Min Jie Huang Brian J Day Abstract Background Cystic fibrosis is a debilitating lung disease due to mutations in the cystic fibrosis transmembrane conductance regulator protein CFTR and is associated with chronic infections resulting in elevated myeloperoxidase activity and generation of hypochlorous acid HOCl . CFTR mutations lead to decreased levels of glutathione GSH and thiocyanate SCN in the epithelial lining fluid ELF . Hypertonic saline is used to improve lung function however the mechanism is uncertain. Methods In the present study the effect of GSH and SCN on HOCl-mediated cell injury and their changes in the ELF after hypertonic saline nebulization in wild type WT and CFTR KO mice was examined. CFTR sufficient and deficient lung cells were assessed for GSH SCN and corresponding sensitivity towards HOCl-mediated injury in vitro. Results CFTR - cells had lower extracellular levels of both GSH and SCN and were more sensitive to HOCl-mediated injury. In vivo hypertonic saline increased ELF GSH in the WT and to a lesser extent in the CFTR KO mice but only SCN in the WT ELF. Finally potential protective effects of GSH and SCN at concentrations found in the ELF against HOCl toxicity were examined in vitro. Conclusions While the concentrations of GSH and SCN associated with the WT ELF protect against HOCl toxicity those found in the CFTR KO mice were less sufficient to inhibit cell injury. These data suggests that CFTR has important roles in exporting GSH and SCN which are protective against oxidants and that hypertonic saline .