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Báo cáo y học: " Systems-level comparison of host responses induced by pandemic and seasonal influenza A H1N1 viruses in primary human type I-like alveolar epithelial cells in vitro"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Systems-level comparison of host responses induced by pandemic and seasonal influenza A H1N1 viruses in primary human type I-like alveolar epithelial cells in vitro. | Lee et al. Respiratory Research 2010 11 147 http respiratory-research.eom content 11 1 147 RESPIRATORY RESEARCH RESEARCH Open Access Systems-level comparison of host responses induced by pandemic and seasonal influenza A H1N1 viruses in primary human type I-like alveolar epithelial cells in vitro Cl iLi K IV I pp1 t Rpnpp A Y hpn 1 2t ppnitpr I C-tprrlv3 r-hp ilz-Lin I Pl4 Alpp nil RihpiP5 Cprp RR ppp suki MT Lee Renee WT LhdH Jennifer L Gardy Clieuk kin LU Alan Dl SHioe Laid SR Kang Timothy KW Cheung1 Yi Guan1 Michael CW Chan1 Robert EW Hancock6 Malik JS Peiris1 7 Abstract Background Pandemic influenza H1N1 pdmH1N1 virus causes mild disease in humans but occasionally leads to severe complications and even death especially in those who are pregnant or have underlying disease. Cytokine responses induced by pdmH1N1 viruses in vitro are comparable to other seasonal influenza viruses suggesting the cytokine dysregulation as seen in H5N1 infection is not a feature of the pdmH1N1 virus. However a comprehensive gene expression profile of pdmH1N1 in relevant primary human cells in vitro has not been reported. Type I alveolar epithelial cells are a key target cell in pdmH1N1 pneumonia. Methods We carried out a comprehensive gene expression profiling using the Affymetrix microarray platform to compare the transcriptomes of primary human alveolar type I-like alveolar epithelial cells infected with pdmH1N1 or seasonal H1N1 virus. Results Overall we found that most of the genes that induced by the pdmH1N1 were similarly regulated in response to seasonal H1N1 infection with respect to both trend and extent of gene expression. These commonly responsive genes were largely related to the interferon IFN response. Expression of the type III IFN IL29 was more prominent than the type I IFN IFN 3 and a similar pattern of expression of both IFN genes was seen in pdmH1N1 and seasonal H1N1 infection. Genes that were significantly down-regulated in response to seasonal H1N1 but not in .