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Báo cáo y học: " Biomechanical modulation of collagen fragment-induced anabolic and catabolic activities in chondrocyte/agarose constructs"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Biomechanical modulation of collagen fragment-induced anabolic and catabolic activities in chondrocyte/agarose constructs. | Chowdhury et al. Arthritis Research Therapy 2010 12 R82 http arthritis-research.eom content 12 3 R82 RESEARCH ARTICLE Open Access Biomechanical modulation of collagen fragment-induced anabolic and catabolic activities in chondrocyte agarose constructs Tina TChowdhury 1 Ronny M Schulz2 Sonpreet S Rai1 Christian BThuemmler2 Nico Wuestneck2 Augustinus Bader2 and Gene A Homandberg3 Abstract Introduction The present study examined the effect of collagen fragments on anabolic and catabolic activities by chondrocyte agarose constructs subjected to dynamic compression. Methods Constructs were cultured under free-swelling conditions or subjected to continuous and intermittent compression regimes in the presence of the N-terminal NT and C-terminal CT telopeptides derived from collagen type II and or 1400 W inhibits inducible nitric oxide synthase iNOS . The anabolic and catabolic activities were compared to the amino-terminal fibronectin fragment NH2-FN-f and assessed as follows nitric oxide NO release and sulphated glycosaminoglycan sGAG content were quantified using biochemical assays. Tumour necrosis factor-a TNFa and interleukin-1p IL-1P release were measured by ELISA. Gene expression of matrix metalloproteinase-3 MMP-3 matrix metalloproteinase-13 MMP-13 collagen type II and fibronectin were assessed by real-time quantitative polymerase chain reaction qPCR . Two-way ANOVA and the post hoc Bonferroni-corrected t-test was used to examine data. Results The presence of the NT or CT peptides caused a moderate to strong dose-dependent stimulation of NO TNFa and IL-1 p production and inhibition of sGAG content. In some instances high concentrations of telopeptides were just as potent in stimulating catabolic activities when compared to NH2-FN-f. Depending on the concentration and type of fragment the increased levels of NO and cytokines were inhibited with 1400 W resulting in the restoration of sGAG content. Depending on the duration and type of compression regime employed .