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Báo cáo y học: " IL-4 and IL-13 exposure during mucociliary differentiation of bronchial epithelial cells increases antimicrobial activity and expression of antimicrobial peptides"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: " IL-4 and IL-13 exposure during mucociliary differentiation of bronchial epithelial cells increases antimicrobial activity and expression of antimicrobial peptides. | Zuyderduyn et al. Respiratory Research 2011 12 59 http respiratory-research.eom content 12 1 59 RESPIRATORY RESEARCH RESEARCH Open Access IL-4 and IL-13 exposure during mucociliary differentiation of bronchial epithelial cells increases antimicrobial activity and expression of antimicrobial peptides Suzanne Zuyderduyn1 3 Dennis K Ninaber1 Jasmijn A Schrumpf1 Marianne AJA van Sterkenburg1 12 1.3 1 1 Renate M Verhoosel Frans A Prins Sandra van Wetering Klaus F Rabe and Pieter S Hiemstra Abstract The airway epithelium forms a barrier against infection but also produces antimicrobial peptides AMPs and other inflammatory mediators to activate the immune system. It has been shown that in allergic disorders Th2 cytokines may hamper the antimicrobial activity of the epithelium. However the presence of Th2 cytokines also affects the composition of the epithelial layer which may alter its function. Therefore we investigated whether exposure of human primary bronchial epithelial cells PBEC to Th2 cytokines during mucociliary differentiation affects expression of the human cathelicidin antimicrobial protein hCAP18 LL-37 and human beta defensins hBD and antimicrobial activity. PBEC were cultured at an air-liquid interface ALI for two weeks in the presence of various concentrations of IL-4 or IL-13. Changes in differentiation and in expression of various AMPs and the antimicrobial proteinase inhibitors secretory leukocyte protease inhibitor SLPI and elafin were investigated as well as antimicrobial activity. IL-4 and IL-13 increased mRNA expression of hCAP18 LL-37 and hBD-2. Dot blot analysis also showed an increase in hCAP18 LL-37 protein in apical washes of IL-4-treated ALI cultures whereas Western Blot analysis showed expression of a protein of approximately 4.5 kDa in basal medium of IL-4-treated cultures. Using sandwich ELISA we found that also hBD-2 in apical washes was increased by both IL-4 and IL-13. SLPI and elafin levels were not affected by IL-4 or IL-13 at the mRNA