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Báo cáo y học: " Ultrastructural changes of the intracellular surfactant pool in a rat model of lung transplantation-related events"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:" Ultrastructural changes of the intracellular surfactant pool in a rat model of lung transplantation-related events. | Knudsen et al. Respiratory Research 2011 12 79 http respiratory-research.eom content 12 1 79 RESPIRATORY RESEARCH RESEARCH Open Access Ultrastructural changes of the intracellular surfactant pool in a rat model of lung transplantation-related events I Tire IZm irlccitì1 t I I 71 Hl ilHH A i z 2t I Iciifi pQhrQtiH r-H3 Dir-Hfor4 TH rc on A tỉ H lore5 TH rcfor A ii f A or5 Lais Knudsen Hdzibulldh Wdizy Heinz Feinenbacn Joachim Richter llioistell wallers llioistell Vvlttwel and Mattllds Ochs1 Abstract Background Isclemid reperfusion I R injury involved in primary graft dysfunction following lung transplantation leads to inactivation of intra-alveolar surfactant which facilitates injury of the blood-air barrier. The alveolar epitlelidl type II cells AE2 cells synthesize store and secrete surfactant thus an intracellular surfactant pool stored in lamellar bodies Lb can be distinguisled from the intra-alveolar surfactant pool. The aim of tlis study was to investigate ultrastructural alterations of the intracellular surfactant pool in a model mimicking transplantation-related procedures including flusl perfusion cold isclemia and reperfusion combined witl meclanical ventilation. Methods Using design-based stereology at the liglt and electron microscopic level number surface area and mean volume of AE2 cells as well as number size and total volume of Lb were determined in a group subjected to transplantation-related procedures including both I R injury and mechanical ventilation I R group and a control group. Results After I R injury tle mean number of Lb per AE2 cell was significantly reduced compared to tle control group accompanied by a significant increase in tle luminal surface area per AE2 cell in tle I R group. Tlis increase in tle luminal surface area correlated witl tle decrease in surface area of Lb per AE2. Tle number-weiglted mean volume of Lb in tle I R group slowed a tendency to increase. Conclusion We suggest tlat in tlis animal model tle reduction of tle .