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Báo cáo y học: "Toll-like receptor 3 upregulation in macrophages participates in the initiation and maintenance of pristane-induced arthritis in rats"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Toll-like receptor 3 upregulation in macrophages participates in the initiation and maintenance of pristane-induced arthritis in rats. | Meng et al. Arthritis Research Therapy 2010 12 R103 http arthritis-research.eom content 12 3 R103 RESEARCH ARTICLE Open Access Toll-like receptor 3 upregulation in macrophages participates in the initiation and maintenance of pristane-induced arthritis in rats Liesu Meng21 2 Wenhua Zhu21 2 Congshan Jiang 1 2 Xiaojing He1 2 Weikun Hou1 2 Fang Zheng1 2 Rikard Holmdahl3 and Shemin Lu 1 2 Abstract Introduction Toll-like receptors TLRs are involved in both innate and adaptive immune responses and are likely to play a complex role in the pathogenesis of human rheumatoid arthritis RA and experimental arthritis. The objective of this study was to identify the key TLR in pristane-induced arthritis PIA a rat model for RA and to clarify its roles in the initiation and maintenance of arthritis. Methods Arthritis in DA rats was induced by pristane and the severity was evaluated by macroscopic and microscopic score systems. Spleen TLR and cytokine expression was detected at different time points by real-time polymerase chain reaction PCR and flow cytometry. Polyinosine-polycytidylic acid polyI C a ligand of TLR3 or TLR3 specific shorthairpin RNA plasmid for RNA interference was administrated to PIA rats in vivo. Serum nitrogen oxide concentration was determined by Griess method and tumor necrosis factor alpha TNF-a was determined by L929 biotest. In splenic macrophages TLR3 expression was measured by flow cytometry. A rat macrophage cell line NR8383 was stimulated by pristane and anti-TLR3 antibody were used to block TLR3 pathway. TLR3 and cytokine expression in NR8383 were detected by real-time PCR. Results By screening the TLR expression profile in spleen of DA rats after pristane injection we found that TLR3 was the most early and prominently upregulated TLR. Both TLR3 mRNA and protein expression of spleen were upregulated at 6 and 26 days after pristane injection. Furthermore administration of polyI C exacerbated whereas RNA interference targeting TLR3 ameliorated the .