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Báo cáo y học: " IL-13 induces a bronchial epithelial phenotype that is profibrotic"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: " IL-13 induces a bronchial epithelial phenotype that is profibrotic. | Respiratory Research BioMed Central Research IL-13 induces a bronchial epithelial phenotype that is profibrotic Nikita K Malavia1 Justin D Mih2 Christopher B Raub2 Bao T Dinh2 and Steven C George 1 2 Open Access Address Department of Chemical Engineering and Materials Science University of California - Irvine Irvine CA USA and 2Department of Biomedical Engineering University of California - Irvine Irvine CA USA Email Nikita K Malavia - nmalavia@uci.edu Justin D Mih - justozero@gmail.com Christopher B Raub - craub@uci.edu Bao T Dinh - btdinh@uci.edu Steven C George - scgeorge@uci.edu Corresponding author Published 18 March 2008 Received 27 December 2007 Respiratory Research 2008 9 27 doi 10.1186 1465-9921 -9-27 Accepted 18 March 2008 This article is available from http respiratory-research.cOm content 9 1 27 2008 Malavia et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Inflammatory cytokines e.g. IL-13 and mechanical perturbations e.g. scrape injury to the epithelium release profibrotic factors such as TGF-P2 which may in turn stimulate subepithelial fibrosis in asthma. We hypothesized that prolonged IL-13 exposure creates a plastic epithelial phenotype that is profibrotic through continuous secretion of soluble mediators at levels that stimulate subepithelial fibrosis. Methods Normal human bronchial epithelial cells NHBE were treated with IL-13 0 0.1 1 or 10 ng ml for 14 days day 7 to day 21 following seeding at an air-liquid interface during differentiation and then withdrawn for 1 or 7 days. Pre-treated and untreated NHBE were cocultured for 3 days with normal human lung fibroblasts NHLF embedded in rat-tail collagen gels during days 22-25 or days 28-31. Results IL-13 induced increasing levels of