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Báo cáo y học: " Microarray identifies ADAM family members as key responders to TGF-β1 in alveolar epithelial cells"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: " Microarray identifies ADAM family members as key responders to TGF-β1 in alveolar epithelial cells. | Respiratory Research BioMed Central Research Open Access Microarray identifies ADAM family members as key responders to TGF-pl in alveolar epithelial cells Dominic T Keating1 2 Denise M Sadlier1 Andrea Patricelli1 Sinead M Smith3 Dermot Walls3 Jim J Egan2 and Peter P Doran 1 Address 1General Clinical Research Unit Mater Misericordiae University Hospital School of Medicine and Medical Sciences University College Dublin Dublin 7 Ireland 2Advanced Lung Disease Programme and Lung Transplant Unit Mater Misericordiae University Hospital and 3School of Biotechnology Dublin City University Dublin Ireland Email DominicT Keating - dkeating@mater.ie Denise M Sadlier - dsadlier@partners.org Andrea Patricelli - apatricelli@mater.ie Sinead M Smith - smithsi@tcd.ie Dermot Walls - dermot.walls@dcu.ie Jim J Egan - jegan@mater.ie Peter P Doran - pdoran.genome@mater.ie Corresponding author Published 01 September 2006 Received 29 May 2006 Accepted 01 September 2006 Respiratory Research 2006 7 114 doi l0.ll 86 1465-9921-7-1 14 This article is available from http respiratory-research.cOm content 7 1 1 14 2006 Keating et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract The molecular mechanisms of Idiopathic Pulmonary Fibrosis IPF remain elusive. Transforming Growth Factor beta Ỉ TGF-PỈ is a key effector cytokine in the development of lung fibrosis. We used microarray and computational biology strategies to identify genes whose expression is significantly altered in alveolar epithelial cells A549 in response to TGF-PL IL-4 and IL-13 and Epstein Barr virus. A549 cells were exposed to 10 ng ml TGF-PL IL-4 and IL-13 at serial time points. Total RNA was used for hybridisation to Affymetrix Human Genome U133A microarrays. Each in vitro .