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Báo cáo y học: "Single-step autoantibody profiling in antiphospholipid syndrome using a multi-line dot assay"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài:Single-step autoantibody profiling in antiphospholipid syndrome using a multi-line dot assay. | Egerer et al. Arthritis Research Therapy 2011 13 R118 http arthritis-research.eom content 13 4 R118 RESEARCH ARTICLE Open Access Single-step autoantibody profiling in antiphospholipid syndrome using a multi-line dot assay 1 t 24t 2 1 1 Karl Egerer Dirk Roggenbuck Thomas Buttner Barbara Lehmann Annushka Kohn Philipp von Landenberg3 Rico Hiemann4 Eugen Feist1 Gerd-Rudiger Burmester1 and Thomas Dorner1 Abstract Introduction Diagnosis of antiphospholipid syndrome APS still remains a laboratory challenge due to the great diversity of antiphospholipid antibodies aPL and their significance regarding APS-diagnostic criteria. Methods A multi-line dot assay MLDA employing phosphatidylserine PS phosphatidylinositol PI cardiolipin CL and beta2-glycoprotein I p2 GPI was used to detect aPL immunoglobulin G IgG and immunoglobulin M IgM in 85 APS patients 65 disease controls and 79 blood donors. For comparison anti-CL and anti-p2 GPI IgG and IgM were detected by enzyme-linked immunosorbent assay ELISA . Results The level of agreement of both methods was good for anti-CL IgG moderate for anti-CL IgM very good for anti-p2 GPI IgG and moderate for anti-p2 GPI IgM kappa 0.641 0.507 0.803 and 0.506 respectively . The frequency of observed discrepancies for anti-CL IgG 1.75 anti-CL IgM 3.93 anti-p2 GPI IgG 1.75 and anti-p2 GPI IgM 0.87 was low McNemar test P 0.05 not-significant respectively . Sensitivity specificity positive LR and negative -LR likelihood ratios for at least one positive aPL antibody assessed by ELISA were 58.8 95.8 14.1 and 0.4 respectively and for at least three positive aPl IgM and or one positive aPL IgG by MLDA were 67.1 96.5 19.3 and 0.3 respectively. The frequency of IgM to PI PS and CL and combination of three or more aPL IgM detected by MLDA was significantly higher in APS patients with cerebral transient ischemia P 0.05 respectively . Conclusions The novel MLDA is a readily available single-step sensitive diagnostic tool for the multiplex detection of aPL .