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Báo cáo y học: " Investigating a pathogenic role for TXNDC5 in rheumatoid arthritis"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Investigating a pathogenic role for TXNDC5 in rheumatoid arthritis. | Chang et al. Arthritis Research Therapy 2011 13 R124 http arthritis-research.eom content 13 4 R124 RESEARCH ARTICLE Open Access Investigating a pathogenic role for TXNDC5 in rheumatoid arthritis Xiaotian Chang1 Yan Zhao1 Xinfeng Yan2 Jihong Pan1 Kehua Fang1 and Lin Wang1 Abstract Introduction Expression of TXNDC5 which is induced by hypoxia stimulates cell proliferation and angiogenesis. Our previous study detected increased TXNDC5 expression in the synovial tissues of rheumatoid arthritis RA patients using proteomic methods. The current study investigated a pathogenic role for TXNDC5 in RA. Method Expression of TXNDC5 in synovial membranes was quantitatively analyzed by immunohistochemistry Western blotting and real-time polymerase chain reaction PCR . Serum TXNDC5 levels and serum anti-TXNDC5 antibody levels were determined using sandwich enzyme-linked immunosorbent assay ELISA . A total of 96 single nucleotide polymorphisms SNPs in or near the TXNDC5 gene were genotyped using custom-designed Illumina 96-SNP VeraCode microassay. Allele frequencies and genotype frequencies of SNPs were assessed using a casecontrol design in a cohort of 267 Chinese patients with RA 51 patients with ankylosing spondylitis AS and 160 healthy controls. Additional genotyping of 951 patients with RA and 898 healthy controls was performed for four SNPs rs2277105 rs369086 rs443861 and rs11962800 using the TaqMan method. Results Real-time PCR Western blotting and immunohistochemistry detected significantly higher TXNDC5 expression in the synovial tissues of RA patients compared to samples from patients with osteoarthritis OA or AS. ELISA detected significantly higher levels of TXNDC5 in the blood of RA patients compared to OA AS and systemic lupus erythematosus patients and healthy controls. ELISA did not detect significantly different levels of anti-TXNDC5 antibody in the blood of RA OA and AS patients and healthy controls. A total of 9 SNPs rs9505298 rs41302895 rs1225936 rs1225938 .