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Báo cáo y học: " Collaborative interactions between neutrophil elastase and metalloproteinases in extracellular matrix degradation in threedimensional collagen gels"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Collaborative interactions between neutrophil elastase and metalloproteinases in extracellular matrix degradation in threedimensional collagen gels. | Available online http respiratory-research.eom content 2 5 300 Research article Collaborative interactions between neutrophil elastase and metalloproteinases in extracellular matrix degradation in threedimensional collagen gels Yunkui Zhu Xiangde Liu C Magnus Skold Hangjun Wang Tadashi Kohyama Fu-Qiang Wen Ronald F Ertl and Stephen I Rennard University of Nebraska Medical Center Omaha Nebraska USA Karolinska Hospital Stockholm Sweden Mount Sinai Hospital Pathology and Laboratory Medicine Toronto Ontario Canada Correspondence Stephen I Rennard MD University of Nebraska Medical Center 985125 Nebraska Medical Center Omaha Nebraska 68198-5125 USA. Tel 1 402 559 7313 fax 1 402 559 4878 e-mail srennard@unmc.edu Received 5 March 2001 Revisions requested 14 May 2001 Revisions received 13 July 2001 Accepted 8 August 2001 Published 4 September 2001 Respir Res 2001 2 300-305 2001 Zhu et al licensee BioMed Central Ltd Print ISSN 1465-9921 Online ISSN 1465-993X Abstract Background Extended culture of monocytes and fibroblasts in three-dimensional collagen gels leads to degradation of the gels see linked study in this issue Fibroblasts and monocytes contract and degrade three-dimensional collagen gels in extended co-culture . The current study therefore was designed to evaluate production of matrix-degrading metalloproteinases by these cells in co-culture and to determine if neutrophil elastase could collaborate in the activation of these enzymes. Since cocultures produce prostaglandin E2 PGE2 the role of PGE2 in this process was also evaluated. Methods Blood monocytes from healthy donors and human fetal lung fibroblasts were cast into type I collagen gels and maintained in floating cultures for three weeks. Matrix metalloproteinases MMPs were assessed by gelatin zymography MMPs 2 and 9 and immunoblotting MMPs 1 and 3 . The role of PGE2 was explored by direct quantification and by the addition of exogenous indomethacin and or PGE2. Results Gelatin zymography and immunoblots .