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Báo cáo y học: "Atorvastatin reduces lipopolysaccharide-induced expression of cyclooxygenase-2 in human pulmonary epithelial cells"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: "Atorvastatin reduces lipopolysaccharide-induced expression of cyclooxygenase-2 in human pulmonary epithelial cells. | Respiratory Research BioMed Central Research Open Access Atorvastatin reduces lipopolysaccharide-induced expression of cyclooxygenase-2 in human pulmonary epithelial cells ShangJie Wu 1 Shu Duan1 ShuiPing Zhao1 Ying Cai2 Ping Chen2 and Xiang Fang3 Address 1Division of Cardiovascular Disease Department of Internal Medicine The Second Xiangya Hospital Center Southern University Changsha Hunan China 2Division of Respiratory Disease Department of Internal Medicine The Second Xiangya Hospital Center Southern University Changsha Hunan China and 3Department of Biochemistry University of Iowa Carver College of Medicine Iowa City IA 52242 USA Email ShangJie Wu - wu_shangjie@hotmail.com Shu Duan - duanshu321@hotmail.com ShuiPing Zhao - zhaosp@medmail.com.cn Ying Cai - caiying5540@sina.com Ping Chen - chenping101@hotmail.com Xiang Fang - xiang-fang@uiowa.edu Corresponding author Published 03 April 2005 Received 20 November 2004 Respiratory Research 2005 6 27 doi 10.1186 1465-9921 -6-27 Accepted 03 April 2005 This article is available from http respiratory-research.cOm content 6 1 27 2005 Wu et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Objective To explore the effects of atorvastatin on expression of cyclooxygenase-2 COX-2 in human pulmonary epithelial cells A549 . Methods A549 cells were incubated in DMEM medium containing lipopolysaccharide LPS in the presence or absence of atorvastatin. After incubation the medium was collected and the amount of prostaglandin E2 PGE2 was measured by enzyme-linked immunosorbent assay ELISA . The cells were harvested and COX-2 mRNA and protein were analyzed by RT-PCR and western-blot respectively. Results LPS increased the expression of COX-2 mRNA and production of PGE2 in a dose- .