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Báo cáo y học: "E1A expression dysregulates IL-8 production and suppresses IL-6 production by lung epithelial cells"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:"E1A expression dysregulates IL-8 production and suppresses IL-6 production by lung epithelial cells. | Respiratory Research BioMed Central Research Open Access EIA expression dysregulates IL-8 production and suppresses IL-6 production by lung epithelial cells Arjen van den Berg 1 2 Mieke Snoek1 2 Henk M Jansen1 and René Lutter1 2 Address department of Pulmonology Academic Medical Center University of Amsterdam Amsterdam The Netherlands and department of Experimental Immunology Academic Medical Center University of Amsterdam Amsterdam The Netherlands Email Arjen van den Berg - a.vandenberg@amc.uva.nl Mieke Snoek - m.snoek@amc.uva.nl Henk M Jansen - h.m.jansen@amc.uva.nl René Lutter - R.Lutter@amc.uva.nl Corresponding author Published 26 September 2005 Received 16 June 2005 Accepted 26 September 2005 Respiratory Research 2005 6 111 doi 10.1186 1465-9921-6-1 II This article is available from http respiratory-research.cOm content 6 1 1 1 1 2005 van den Berg et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The adenoviral protein E1A has been proposed to play a role in the pathophysiology of COPD in particular by increasing IL-8 gene transcription of lung epithelial cells in response to cigarette smoke-constituents such as LPS. As IL-8 production is also under tight post-transcriptional control we planned to study whether E1A affected IL-8 production post-transcriptionally. The production of IL-6 by E1A-positive cells had not been addressed and was studied in parallel. Based on our previous work into the regulation of IL-8 and IL-6 production in airway epithelial cells we used the lung epithelial-like cell line NCI-H292 to generate stable transfectants expressing either E1A and or E1B which is known to frequently co-integrate with E1A. We analyzed IL-8 and IL-6 production and the underlying regulatory .