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Báo cáo khoa học: " In vitro analysis of expression vectors for DNA vaccination of horses: the effect of a Kozak sequence"

Tuyển tập các báo cáo nghiên cứu về bệnh học thý y được đăng trên tạp chí Acta Veterinaria Scandinavica cung cấp cho các bạn kiến thức về bệnh thú y đề tài: In vitro analysis of expression vectors for DNA vaccination of horses: the effect of a Kozak sequence. | Acta Veterinaria Scandinavica BioMed Central Research In vitro analysis of expression vectors for DNA vaccination of horses the effect of a Kozak sequence Guổbjổrg Ólaísdóttir1 Vilhjálmur Svansson 1 Sigurổur Ingvarsson1 Eliane Marti2 and Sigurbjổrg Torsteinsdóttir1 Open Access Address 1Institute for Experimental Pathology University of Iceland Keldur Reykjavik Iceland and 2Department of Clinical Veterinary Medicine Vetsuisse Faculty University of Berne Switzerland Email Guổbjổrg Ólafsdóttir - gudbjol@hi.is Vilhjálmur Svansson - vsvanss@hi.is Sigurổur Ingvarsson - siguring@hi.is Eliane Marti - eliane.marti@itz.unibe.ch Sigurbjổrg Torsteinsdóttir - sibbath@hi.is Corresponding author Published 4 November 2008 Received 10 April 2008 Accepted 4 November 2008 Acta Veterinaria Scandinavica 2008 50 44 doi 10.1186 1751-0147-50-44 r This article is available from http www.actavetscand.com content 50 1 44 2008 Ólafsdóttir et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract One of the prerequisite for developing DNA vaccines for horses are vectors that are efficiently expressed in horse cells. We have analysed the ectopic expression of the human serum albumin gene in primary horse cells from different tissues. The vectors used are of pcDNA and pUC origin and include the cytomegalovirus CMV promoter. The pUC vectors contain CMV intron A whereas the pcDNA vectors do not. Insertion of intron A diminished the expression from the pcDNA vectors whereas insertion of a Kozak sequence upstream of the gene in two types of pUC vectors increased significantly the in vitro expression in primary horse cells derived from skin lung duodenum and kidney. We report for the first time the significance of full consensus Kozak sequences for .