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Báo cáo khoa học: "Screening for bovine leukocyte adhesion deficiency, deficiency of uridine monophosphate synthase, complex vertebral malformation, bovine citrullinaemia, and factor XI deficiency in Holstein cows reared in Turkey"

Tuyển tập các báo cáo nghiên cứu về bệnh học thý y được đăng trên tạp chí Acta Veterinaria Scandinavica cung cấp cho các bạn kiến thức về bệnh thú yđề tài: Screening for bovine leukocyte adhesion deficiency, deficiency of uridine monophosphate synthase, complex vertebral malformation, bovine citrullinaemia, and factor XI deficiency in Holstein cows reared in Turkey. | Meydan et al. Acta Veterinaria Scandinavica 2010 52 56 http www.actavetscand.eom content 52 1 56 AVS ACTAVETERINARIA SCANDINAVICA RESEARCH Open Access Screening for bovine leukocyte adhesion deficiency deficiency of uridine monophosphate synthase complex vertebral malformation bovine citrullinaemia and factor XI deficiency in Holstein cows reared in Turkey Hasan Meydan 1 Mehmet A Yildiz1 J0rgen S Agerholm2 Abstract Background Bovine leukocyte adhesion deficiency BLAD deficiency of uridine monophosphate synthase DUMPS complex vertebral malformation CVM bovine citrullinaemia BC and factor XI deficiency FXID are autosomal recessive hereditary disorders which have had significant economic impact on dairy cattle breeding worldwide. In this study 350 Holstein cows reared in Turkey were screened for BLAD DUMPS CVM BC and FXID genotypes to obtain an indication on the importance of these defects in Turkish Holsteins. Methods Genomic DNA was obtained from blood and the amplicons of BLAD DUMPS CVM BC and FXID were obtained by using PCR. PCR products were digested with TaqI AvaI and AvaII restriction enzymes for BLAD DUMPS and BC respectively. These digested products and PCR product of FXID were analyzed by agarose gel electrophoresis stained with ethidium bromide. CVM genotypes were detected by DNA sequencing. Additionally all genotypes were confirmed by DNA sequencing to determine whether there was a mutant allele or not. Results Fourteen BLAD twelve CVM and four FXID carriers were found among the 350 Holstein cows examined while carriers of DUMPS and BC were not detected. The mutant allele frequencies were calculated as 0.02 0.017 and 0.006 for BLAD CVM and FXID respectively with corresponding carrier prevalence of 4.0 BLAD 3.4 CVM and 1.2 FXID . Conclusion This study demonstrates that carriers of BLAD CVM and FXID are present in the Turkish Holstein population although at a low frequency. The actual number of clinical cases is unknown but sporadic cases may appear. As .