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Báo cáo khoa học: "Clonal spread of antimicrobial-resistant Escherichia coli isolates among pups in two kennels"

Tuyển tập các báo cáo nghiên cứu về bệnh học thý y được đăng trên tạp chí Acta Veterinaria Scandinavica cung cấp cho các bạn kiến thức về bệnh thú yđề tài: Clonal spread of antimicrobial-resistant Escherichia coli isolates among pups in two kennels. | Harada et al. Acta Veterinaria Scandinavica 2011 53 11 http www.actavetscand.eom content 53 1 11 AVS ACTAVETERINARIA SCANDINAVICA BRIEF COMMUNICATION Open Access Clonal spread of antimicrobial-resistant Escherichia coli isolates among pups in two kennels Kazuki Harada Erika Morimoto Yasushi Kataoka Toshio Takahashi Abstract Although the dog breeding industry is common in many countries the presence of antimicrobial resistant bacteria among pups in kennels has been infrequently investigated. This study was conducted to better understand the epidemiology of antimicrobial-resistant Escherichia coli isolates from kennel pups not treated with antimicrobials. We investigated susceptibilities to 11 antimicrobials and prevalence of extended-spectrum p-lactamase ESBL in 86 faecal E. coli isolates from 43 pups in two kennels. Genetic relatedness among all isolates was assessed using pulsed-field gel electrophoresis PFGE . Susceptibility tests revealed that 76 of the isolates were resistant to one or more of tested antimicrobials with resistance to dihydrostreptomycin most frequently encountered 66.3 followed by ampicillin 60.5 trimethoprim-sulfamethoxazole 41.9 oxytetracycline 26.7 and chloramphenicol 26.7 . Multidrug resistance defined as resistance against two or more classes of antimicrobials was observed in 52 60.5 isolates. Three pups in one kennel harboured SHV-12 ESBL-producing isolates. A comparison between the two kennels showed that frequencies of resistance against seven antimicrobials and the variation in resistant phenotypes differed significantly. Analysis by PFGE revealed that clone sharing rates among pups of the same litters were not significantly different in both kennels 64.0 vs. 88.9 whereas the rates among pups from different litters were significantly different between the two kennels 72.0 vs. 33.3 P 0.05 . The pups in the two kennels had antimicrobial-resistant E. coli clones including multidrug-resistant and ESBL-producing clones. It is likely that .